Epidemiological and clinical characteristics of cases and detection of pathogens’ markers of tick-borne infections in the Tomsk region
- 作者: Ilyinskikh E.N.1, Bondarenko E.I.2, Voronkova O.V.1, Karpova M.R.1, Filatova E.N.1, Reshetova A.V.3, Poltoratskaya T.N.4, Sizova N.N.4, Koryakova O.S.4
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隶属关系:
- Siberian State Medical University
- JSC “Vector-Best”
- Siberian State Medical University, Tomsk, Russia
- Center for Hygiene and Epidemiology in the Tomsk Region
- 期: 卷 29, 编号 4 (2024)
- 页面: 260-273
- 栏目: Original study articles
- ##submission.dateSubmitted##: 17.07.2024
- ##submission.dateAccepted##: 31.07.2024
- ##submission.datePublished##: 07.10.2024
- URL: https://rjeid.com/1560-9529/article/view/634387
- DOI: https://doi.org/10.17816/EID634387
- ID: 634387
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详细
BACKGROUND: Among the spectrum of the tick-borne infections, Lyme borreliosis and tick-borne encephalitis are most often recorded in the Tomsk region, however it is obviously that possible range of the pathogens is not limited to these ones.
AIM: To make epidemiological and clinical characteristics of cases and detect frequencies of pathogen’s markers of tick-borne infections among adult patients applying serological and molecular genetic techniques in the Tomsk region during 2020–2023.
METHODS: A single-center prospective sample study was conducted applying a comprehensive approach in peripheral blood specimens obtained from 197 patients with suspected tick-borne infections hospitalized during 2020–2023. To verify the diagnoses of tick-borne encephalitis or Lyme borreliosis, specific immunoglobulins of classes M and G to the antigens of virus of tick-borne encephalitis and Borrelia burgdorferi s.l. were determined in paired blood serum samples applying enzyme-linked immunosorbent assay. Additionally, the polymerase chain reaction was used to detect genetic markers of virus of tick-borne encephalitis, B. burgdorferi s.l., B. miyamotoi, Rickettsia spp., Coxiella burnetii, and Francisella tularensis. Epidemiological surveillance data of incidence rates of tick-borne infections obtained from official reports were analyzed as well. MS Office Excel 2010 software was used to calculate frequency morbidity measures.
RESULTS: We verified the final diagnosis of tick-borne encephalitis in 52 (26.4%) patients, Lyme borreliosis in 116 (58.9%) patients, co-infection of tick-borne encephalitis and Lyme borreliosis 15 (7.6%) patients, hard tick relapsing fever caused by B. miyamotoi in 8 (4.1%) patients, coinfection of Q fever caused by C. burnetii with tick-borne encephalitis or Lyme borreliosis in 4 (2.0%) patients, and coinfection of tularemia with tick-borne encephalitis or Lyme borreliosis in 2 (1.0%) cases. We identified markers of tick-borne encephalitis and Lyme borreliosis pathogens by polymerase chain reaction in 3.6% and 4.1% of the patients. Most number of individuals with tick bites, and the highest incidence rates of Lyme borreliosis and tick-borne encephalitis were recorded in the suburbs of Tomsk. The age groups of 40–59 years, and elderly people 60 years and older were predominated among the patients.
CONCLUSIONS: It was found out that, in addition to Lyme borreliosis and tick-borne encephalitis, there were such tick-borne infections as tick relapsing fever, Q fever, and tularemia in the Tomsk region in the 2020–2023 period.
全文:

作者简介
Ekaterina Ilyinskikh
Siberian State Medical University
编辑信件的主要联系方式.
Email: infconf2009@mail.ru
ORCID iD: 0000-0001-7646-6905
SPIN 代码: 5245-5958
Scopus 作者 ID: 6602611268
Researcher ID: P-1653-2016
MD, Dr. Sci. (Medicine), Assistant Professor
俄罗斯联邦, 2 Moskovsky trakt, 634050 TomskEvgeny Bondarenko
JSC “Vector-Best”
Email: ebond111@yandex.ru
ORCID iD: 0000-0002-4699-9548
SPIN 代码: 2302-9058
MD, Cand. Sci. (Medicine)
俄罗斯联邦, NovosibirskOlga Voronkova
Siberian State Medical University
Email: Voronkova-ov@yandex.ru
ORCID iD: 0000-0001-9478-3429
SPIN 代码: 8005-8110
Scopus 作者 ID: 12773202300
Researcher ID: O-7952-2016
MD, Dr. Sci. (Medicine), Assistant Professor
俄罗斯联邦, 2 Moskovsky trakt, 634050 TomskMaria Karpova
Siberian State Medical University
Email: mrkarpova@mail.ru
ORCID iD: 0000-0002-7109-9955
SPIN 代码: 6935-7447
Scopus 作者 ID: 7006372355
Researcher ID: M-9333-2016
MD, Dr. Sci. (Medicine), Professor
俄罗斯联邦, 2 Moskovsky trakt, 634050 TomskEvgenia Filatova
Siberian State Medical University
Email: synamber@mail.ru
ORCID iD: 0000-0001-9951-8632
SPIN 代码: 8094-3417
Researcher ID: AEQ-2635-2022
MD
俄罗斯联邦, 2 Moskovsky trakt, 634050 TomskAlina Reshetova
Siberian State Medical University, Tomsk, Russia
Email: wind_of_change95@mail.ru
ORCID iD: 0000-0001-5195-3897
SPIN 代码: 2690-1166
Researcher ID: ACK-7745-2022
MD
俄罗斯联邦, 2, Moskovsky trakt, Tomsk, 634050, RussiaTatyana Poltoratskaya
Center for Hygiene and Epidemiology in the Tomsk Region
Email: ptn077@bk.ru
ORCID iD: 0000-0002-1992-4970
SPIN 代码: 4478-0309
MD, Cand. Sci. (Medicine)
俄罗斯联邦, TomskNatalya Sizova
Center for Hygiene and Epidemiology in the Tomsk Region
Email: parsesto@cge.tom.ru
ORCID iD: 0009-0004-2528-1125
MD
俄罗斯联邦, TomskOlga Koryakova
Center for Hygiene and Epidemiology in the Tomsk Region
Email: koryakova.o.s@cge.tom.ru
ORCID iD: 0009-0004-4092-589X
MD
俄罗斯联邦, Tomsk参考
- On the state of sanitary and epidemiological well-being of the population in the Russian Federation in 2023: State Report. Moscow: Federal’naja sluzhba po nadzoru v sfere zashhity prav potrebitelej i blagopoluchija cheloveka; 2024. 364 p. (In Russ.) Available from: https://www.rospotrebnadzor.ru/documents/details.php?ELEMENT_ID=27779&ysclid=ly1s26kenb761505960
- Poltoratskaya NV, Poltoratskaya TN, Pankina TM, et al. Analysis of the epidemiological situation on tick-borne encephalitis and ixodid tick-borne borreliosis in Tomsk region. Medical Parasitology and Parasitic Diseases. 2021;(1):9–15. (In Russ.) doi: 10.33092/0025-8326mp2021.1.9-15
- Voronkova OV, Romanenko VN, Simakova AV, et al. Analysis of multiple infection in ixodic ticks Dermacentor reticulatus in a combined natural focus of vector-borne infections in the Tomsk region. Problems of Particularly Dangerous Infections. 2023;(2): 106–111. (In Russ.) doi: 10.21055/0370-1069-2023-2-106-111
- Voronkova OV, Lukashova LV, Karpova MR, et al. Distribution of the causative agent of relapsing tick-borne fever Borrelia miyamotoi in natural focus in the Tomsk region. Epidemiology and Infectious Diseases. 2022;27(1):15–22. (In Russ.) doi: 10.17816/EID109070
- Kondranova AM, Karpova MR, Bondarenko EI, et al. Pervyi podtverzhdennyi sluchai kleshchevoi vozvratnoi likhoradki, vyzvannyi Borrelia miyamotoi, v Tomskoi oblasti [abstract]. Russian Journal of Infection and Immunity. 2017;(S):450. (In Russ.)
- Platonov AE, Karan LS, Kolyasnikova NM, et al. Humans infected with relapsing fever spirochete Borrelia miyamotoi, Russia. Emerg Infect Dis. 2011;17(10):1816–1823. doi: 10.3201/eid1710.101474
- Platonov AE, Toporkova MG, Kolyasnikova NM, et al. Clinical presentation of Ixodes tick-borne borreliosis caused by Borrelia miyamotoi in the context of an immune response to the pathogen. Terapevticheskii arkhiv. 2017;89(11):35–43. (In Russ.) doi: 10.17116/terarkh2017891135-43
- Krasnova EI, Savelyeva MV, Khokhlova NI, et al. Features of clinical manifestations and laboratory diagnosis of tick-borne relapsing fever caused by Borrelia miyamotoi in the Novosibirsk Region. Èpidemiologiâ i Infekcionnye Bolezni. Aktual’nye voprosy. 2017;(2):10–15. (In Russ.) EDN: YMVLMJ
- Del Cerro A, Oleaga A, Somoano A, et al. Molecular identification of tick-borne pathogens (Rickettsia spp., Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Coxiella burnetii and piroplasms) in questing and feeding hard ticks from North-Western Spain. Ticks Tick Borne Dis. 2022;13(4):101961. doi: 10.1016/j.ttbdis.2022.101961
- Raileanu C, Moutailler S, Pavel I, et al. Borrelia diversity and co-infection with other tick borne pathogens in ticks. Front Cell Infect Microbiol. 2017;(7):36. doi: 10.3389/fcimb.2017.00036
- Yakovchits NV, Andaev EI, Bondarenko EI, et al. Evaluation of diagnostic significance of complex detection of the tick-borne infection markers in the patients suffered from its bites. Infectious Diseases: News, Opinions, Training. 2016;(4(17)):58–65. (In Russ.) EDN: XHCVIX
- Ierusalimsky AP. Tick-borne encephalitis. Guidelines for physicians. Novosibirsk: Gosudarstvennaya meditsinskaya akademiya MZ RF; 2001. 360 p. (In Russ.)
- Lobzin YuV, Uskov AN, Kozlov SS. Laim-borrelioz: iksodovye kleshchevye borreliozy. Saint Peterburg: Foliant; 2000. 160 p. (In Russ.)
- Bondarenko EI, Filimonova ES, Krasnova EI, et al. Cases of Q fever detected in residents of the Novosibirsk region hospitalized with suspection of infections transmitted by ticks. Russian Clinical Laboratory Diagnostics. 2021;66(4):229–236. (In Russ.) doi: 10.51620/0869-2084-2021-66-4-229-236
- Hansford KM, Wheeler BW, Tschirren B, Medlock JM. Questing Ixodes ricinus ticks and Borrelia spp. in urban green space across Europe: A review. Zoonoses Public Health. 2022;69(3):153–166. doi: 10.1111/zph.12913
- Andronova NV, Minoranskaya NS, Minoranskaya EI. The specific immune response and some remote results in the acute course of tick-borne borreliosis and mixed-infection of tick-borne encephalitis and tick-borne borreliosis. Sibirskii meditsinskii zhurnal (Irkutsk). 2011;100(1):54–57. (In Russ.) EDN: NQVIHT
- Branda JA, Steere AC. Laboratory diagnosis of Lyme borreliosis. Clin Microbiol Rev. 2021;34(2):e00018–e00019. doi: 10.1128/CMR.00018-19
- Uskov AN, Lobzin YuV, Burgasova OA. Tick-borne encephalitis, ehrlichiosis, babesiosis and other topical tick-borne infections in Russia. Infektsionnye Bolezni. 2010;8(2):83–88. (In Russ.) EDN: NRACEN
- Tkachev SE, Fomenko NV, Rar VA, et al. PCR detection and molecular-genetic analysis of tick-transmitted pathogens in patients of Novosibirsk region, Russia. Int J Med Microbiol. 2008; 298 Suppl. 1:365–367. doi: 10.1016/j.ijmm.2007.12.010
- Filimonova ES, Bondarenko EI, Krasnova EI, et al. Detection of agents of natural foci infections. Infectious Diseases: News, Opinions, Training. 2021;10(4):90–97. (In Russ.) doi: 10.33029/2305-3496-2021-10-4-90-97
- Cleveland DW, Anderson CC, Brissette CA. Borrelia miyamotoi: a comprehensive review. Pathogens. 2023;12(2):267. doi: 10.3390/pathogens12020267
- Sokirkina EN, Pichurina NL, Noskov AK. Epidemiological situation on Q fever in the Russian Federation (during years 2013–2022). The Far Eastern Journal of Infectious Pathology. 2023;(45):59–64. (In Russ.) EDN: LYIATA
- Ullah Q, Jamil T, Saqib M, et al. Q Fever — a neglected zoonosis. Microorganisms. 2022;10(8):1530. doi: 10.3390/microorganisms10081530
- Stanko M, Derdáková M, Špitalská E, Kazimírová M. Ticks and their epidemiological role in Slovakia: from the past till present. Biologia (Bratislava). 2022;77(6):1575–1610. doi: 10.1007/s11756-021-00845-3
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