In vitro study of the bactericidal activity for new derivatives of quaternary ammonium salts based on pyridoxine against gram-positive / gram-negative bacteria
- 作者: Agafonova M..1, Bulatova E..1, Sapozhnikov S.V.1, Biktimirova A.S.1, Kobylinskaia E.D.1, Shtyrlin N.V.1, Shtyrlin Y.G.1
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隶属关系:
- Kazan Federal University
- 期: 卷 29, 编号 2 (2024)
- 页面: 118-125
- 栏目: Original study articles
- ##submission.dateSubmitted##: 20.12.2023
- ##submission.dateAccepted##: 13.03.2024
- ##submission.datePublished##: 10.06.2024
- URL: https://rjeid.com/1560-9529/article/view/624847
- DOI: https://doi.org/10.17816/EID624847
- ID: 624847
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BACKGROUND: Preventive care and successful treatment of infectious diseases (including hospital-acquired infections) are among the most important tasks of modern healthcare. Not many effective antiseptics and disinfectants available can solve this problem. Quaternary ammonium salts are currently one of the promising classes of disinfectants; however, their use is limited by the ability of microorganisms to develop resistance to the compounds. Modification of quaternary ammonium salts with substituents of various profiles is a possible way to solve this problem.
AIM: To conduct a comparative analysis of the bactericidal activity of two original quaternary ammonium compounds based on pyridoxine in comparison with benzalkonium chloride.
MATERIALS AND METHODS: The bactericidal profiles of 0.1 and 0.2% aqueous solutions of original quaternary ammonium salts based on pyridoxine were studied against two widespread pathogens: Staphylococcus aureus and Escherichia coli. Antibacterial activity data were obtained using a standard in vitro test (bactericidal activity test on a metal surface; exposure time of 1, 5, and 15 min). In addition to standard conditions, antibacterial efficacy was studied under protein loading conditions, in which BSA solution was added to the surface.
RESULTS: For the studied compounds, the reduction factor (RF, reduction lgCFU of microorganisms) was on average ≥3. Protein loading led to a decrease in efficiency by 1–3 times. An interesting effect of exposure time-dependent specificity reversal to the S. aureus and E. coli was observed.
CONCLUSIONS: The results showed the high activity of the studied compounds, which corresponds to the effectiveness criterion of RF ≥3 for commercial compounds used in this field.
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作者简介
Mariya Agafonova
Kazan Federal University
编辑信件的主要联系方式.
Email: Mariya.Agafonova@kpfu.ru
ORCID iD: 0000-0001-5286-4116
SPIN 代码: 9019-9731
Cand. Sci. (Chemistry)
俄罗斯联邦, KazanElena Bulatova
Kazan Federal University
Email: elena_krylova.stud2015@mail.ru
ORCID iD: 0000-0003-1529-1018
SPIN 代码: 8678-3777
俄罗斯联邦, Kazan
Sergey Sapozhnikov
Kazan Federal University
Email: sapozhnikovsergei@gmail.com
ORCID iD: 0000-0003-2864-5620
SPIN 代码: 1648-2576
Cand. Sci. (Chemistry)
俄罗斯联邦, KazanAlina Biktimirova
Kazan Federal University
Email: yandimirova2016@mail.ru
ORCID iD: 0009-0008-2937-9882
SPIN 代码: 9717-0221
俄罗斯联邦, Kazan
Elizaveta Kobylinskaia
Kazan Federal University
Email: EDKobylinskaya@stud.kpfu.ru
ORCID iD: 0009-0002-5982-6459
俄罗斯联邦, Kazan
Nikita Shtyrlin
Kazan Federal University
Email: Nikita.Shtyrlin@kpfu.ru
ORCID iD: 0000-0001-7926-5121
SPIN 代码: 7270-0800
Cand. Sci. (Chemistry)
俄罗斯联邦, KazanYurii Shtyrlin
Kazan Federal University
Email: Yuri.Shtyrlin@kpfu.ru
ORCID iD: 0000-0002-2777-719X
SPIN 代码: 9316-4418
Dr. Sci. (Chemistry)
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