In vitro study of the bactericidal activity for new derivatives of quaternary ammonium salts based on pyridoxine against gram-positive / gram-negative bacteria
- Authors: Agafonova M..1, Bulatova E..1, Sapozhnikov S.V.1, Biktimirova A.S.1, Kobylinskaia E.D.1, Shtyrlin N.V.1, Shtyrlin Y.G.1
-
Affiliations:
- Kazan Federal University
- Issue: Vol 29, No 2 (2024)
- Pages: 118-125
- Section: ORIGINAL STUDIES
- Submitted: 20.12.2023
- Accepted: 13.03.2024
- Published: 10.06.2024
- URL: https://rjeid.com/1560-9529/article/view/624847
- DOI: https://doi.org/10.17816/EID624847
- ID: 624847
Cite item
Abstract
BACKGROUND: Preventive care and successful treatment of infectious diseases (including hospital-acquired infections) are among the most important tasks of modern healthcare. Not many effective antiseptics and disinfectants available can solve this problem. Quaternary ammonium salts are currently one of the promising classes of disinfectants; however, their use is limited by the ability of microorganisms to develop resistance to the compounds. Modification of quaternary ammonium salts with substituents of various profiles is a possible way to solve this problem.
AIM: To conduct a comparative analysis of the bactericidal activity of two original quaternary ammonium compounds based on pyridoxine in comparison with benzalkonium chloride.
MATERIALS AND METHODS: The bactericidal profiles of 0.1 and 0.2% aqueous solutions of original quaternary ammonium salts based on pyridoxine were studied against two widespread pathogens: Staphylococcus aureus and Escherichia coli. Antibacterial activity data were obtained using a standard in vitro test (bactericidal activity test on a metal surface; exposure time of 1, 5, and 15 min). In addition to standard conditions, antibacterial efficacy was studied under protein loading conditions, in which BSA solution was added to the surface.
RESULTS: For the studied compounds, the reduction factor (RF, reduction lgCFU of microorganisms) was on average ≥3. Protein loading led to a decrease in efficiency by 1–3 times. An interesting effect of exposure time-dependent specificity reversal to the S. aureus and E. coli was observed.
CONCLUSIONS: The results showed the high activity of the studied compounds, which corresponds to the effectiveness criterion of RF ≥3 for commercial compounds used in this field.
Full Text
![Restricted Access](https://rjeid.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
Mariya N. Agafonova
Kazan Federal University
Author for correspondence.
Email: Mariya.Agafonova@kpfu.ru
ORCID iD: 0000-0001-5286-4116
SPIN-code: 9019-9731
Cand. Sci. (Chemistry)
Russian Federation, KazanElena S. Bulatova
Kazan Federal University
Email: elena_krylova.stud2015@mail.ru
ORCID iD: 0000-0003-1529-1018
SPIN-code: 8678-3777
Russian Federation, Kazan
Sergey V. Sapozhnikov
Kazan Federal University
Email: sapozhnikovsergei@gmail.com
ORCID iD: 0000-0003-2864-5620
SPIN-code: 1648-2576
Cand. Sci. (Chemistry)
Russian Federation, KazanAlina S. Biktimirova
Kazan Federal University
Email: yandimirova2016@mail.ru
ORCID iD: 0009-0008-2937-9882
SPIN-code: 9717-0221
Russian Federation, Kazan
Elizaveta D. Kobylinskaia
Kazan Federal University
Email: EDKobylinskaya@stud.kpfu.ru
ORCID iD: 0009-0002-5982-6459
Russian Federation, Kazan
Nikita V. Shtyrlin
Kazan Federal University
Email: Nikita.Shtyrlin@kpfu.ru
ORCID iD: 0000-0001-7926-5121
SPIN-code: 7270-0800
Cand. Sci. (Chemistry)
Russian Federation, KazanYurii G. Shtyrlin
Kazan Federal University
Email: Yuri.Shtyrlin@kpfu.ru
ORCID iD: 0000-0002-2777-719X
SPIN-code: 9316-4418
Dr. Sci. (Chemistry)
Russian Federation, KazanReferences
- Tischer M, Pradel G, Ohlsen K, Holzgrabe U. Quaternary Ammonium Salts and Their Antimicrobial Potential: Targets or Nonspecific Interactions? ChemMedChem. 2012;7(1):22–31. doi: 10.1002/cmdc.201100404
- McDonell G, Russel AD. Antiseptics and Disinfectants: Activity, Action and Resistance. Clin Microbiol Rev. 1999;12(1):147–179. doi: 10.1128/CMR.12.1.147 Erratum in: Clin Microbiol Rev. 2001; 14(1):227.
- Gerba CP. Quaternary Ammonium Biocides: Efficacy in Application. Appl Environ Microbiol. 2015;81(2):464–469. doi: 10.1128/AEM.02633-14
- Pereira BMP, Tagkopoulos I. Benzalkonium chlorides: Uses, regulatory status, and microbial resistance. Appl Environ Microbiol. 2019;85(13):e00377-19. doi: 10.1128/AEM.00377-19
- Thomas L, Russell AD, Maillard JY. Antimicrobial activity of chlorhexidine diacetate and benzalkonium chloride against Pseudomonas aeruginosa and its response to biocide residues. J Appl Microbiol. 2005;98(3):533–543. doi: 10.1111/j.1365-2672.2004.02402.x
- BS EN 14885:2015 E Chemical disinfectants and antiseptics ― Application of European Standards for chemical disinfectants and antiseptics [Internet]. Available from: https://standards.iteh.ai/catalog/standards/cen/1b75492a-aade-4295-9fcd-a376dd55f3ae/en-14885-2015
- Müller G, Kramer A. In vitro action of a combination of selected antimicrobial agents and chondroitin sulfate. Chem Biol Interact. 2000(2);124(2):77–85. doi: 10.1016/S0009-2797(99)00142-8
- López-Rojasa R, Fernández-Cuenca F, Serrano-Rocha L, Pascual Á. In vitro activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens. Enferm Infecc Microbiol Clin. 2017;35(1):12–19. doi: 10.1016/j.eimc.2016.02.008
- Koburger T, Hübner NO, Braun M, Siebert J, Kramer A. Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, polyhexanide and chlorhexidine digluconate. Antimicrob Chemother. 2010;65(8): 1712–1719. doi: 10.1093/jac/dkq212
- Shtyrlin Yu.G., Sapozhnikov S.V., Shtyrlin N.V., Biktimirova A.S., Bulatova A.S., Agafonova M.N.; KFU. Quaternary ammonium compounds based on pentaerythritol and pyridoxine, which have antibacterial activity. RF patent No. 2811203. 11 Jan 2024.
- EN 14885:2015 Chemical disinfectants and antiseptics — Application of European Standards for chemical disinfectants and antiseptics [Internet]. Available from: https://standards.iteh.ai/catalog/standards/cen/1b75492a-aade-4295-9fcd-a376dd55f3ae/en-14885-2015
- CSN EN 1499:2013 Chemical disinfectants and antiseptics — Hygienic handwash — Test method and requirements (phase 2/step 2) [Internet]. Available from: https://standards.iteh.ai/catalog/standards/cen/7f7742a1-1f13-46d1-9e15-fddfcd18ffe0/en-1499-2013
- EN 1500:1997 Chemical disinfectants and antiseptics — Hygienic handrub — Test method and requirements (phase 2/step 2) [Internet]. Available from: https://standards.iteh.ai/catalog/standards/cen/f86b1705-62de-45fe-ac52-01d21161d73a/en-1500-1997
- BS EN 13697:2015 Chemical disinfectants and antiseptics — Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas — Test method and requirements without mechanical action (phase 2, step 2) [Internet]. Available from: https://www.wfcascade.com/wp-content/uploads/2020/07/5605-13697-Holquat-60-seconds-4.pdf
- BS EN 13727+A2:2015 Chemical disinfectants and antiseptics — Quantitative suspension test for the evaluation of bactericidal activity in the medical area — Test method and requirements (phase 2, step 1) [Internet]. Available from: https://www.en-standard.eu/bs-en-13727-2012-a2-2015-chemical-disinfectants-and-antiseptics-quantitative-suspension-test-for-the-evaluation-of-bactericidal-activity-in-the-medical-area-test-method-and-requirements-phase-2-step-1/
- CLSI. Methods for Dilution Antimicrobial Susceptibility Tests f or Bacteria That Grow Aerobically. Approved Standard. 9th Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute; 2012. Available from: https://clsi.org/media/1928/m07ed11_sample.pdf
- EN 1040:1997 Chemical disinfectants and antiseptics, Basic bactericidal activity-test method and requirements (phase 1). German version [Internet]. Available from: https://standards.iteh.ai/catalog/standards/cen/7edfa93a-7b9f-4ee7-891c-87b46985f22c/en-1040-1997
- Kim H, Ryu JH, Beuchat Larry R. Effectiveness of Disinfectants in Killing Enterobacter sakazakii in Suspension, Dried on the Surface of Stainless Steel and in a Biofilm. Appl Environ Microbiol. 2007;73(4):1256–1265. doi: 10.1128/AEM.01766-06
- Singh M., Sharma R., Gupta Pramod K., Rana Jatinder K., Sharma M., and Taneja N. Comparative efficacy evaluation of disinfectants routinely used in hospital practice: India. Indian J Crit Care Med. 2012;16(3):123–129. doi: 10.4103/0972-5229.102067
- Hedge A. Survival of Escherichia coli, Pseudomona aeruginosa, Staphylococcus aureus on Wood and Plastic Surfaces. J Microb Biochem Technol. 2015;7(4):210–212. doi: 10.4172/1948-5948.1000207
- Wiegand C, Abel M, Ruth P, Elsner P, Hipler UC. pH Influence on Antibacterial Efficacy of Common Antiseptic Substances. Skin Pharmacol Physiol. 2015;28(3):147–158. doi: 10.1159/000367632
Supplementary files
![](/img/style/loading.gif)