Antimicrobial resistance profile of Staphylococcus aureus and Enterococcus faecalis isolated in the Kyrgyz Republic



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Abstract

BACKGROUND: The dissemination of antibiotic-resistant microorganisms and resistance genes through food products is a serious problem for global public health. In this context, the aim of this study was to conduct surveillance of antibiotic-resistant bacteria isolated from food products in the Kyrgyz Republic by studying their phenotypic and genotypic susceptibility profiles.

AIM: Conducting epidemiological monitoring of antibiotic-resistant bacteria isolated from food products in the Kyrgyz Republic by analyzing their phenotypic and genotypic susceptibility profiles.

METHODS: The objects of the study were antibiotic-resistant bacterial cultures of S.aureus (n=16) and E.faecalis (n=36), isolated from ready-to-eat food products in the Kyrgyz Republic in the period 2020-2023. Identification of microorganisms was carried out using the MALDI-TOF method mass spectrometry. Phenotypic susceptibility was determined in relation to 20 antimicrobial drugs using the minimum inhibitory concentration method. Antimicrobial resistance genes were determined using data analysis of whole-genome sequencing.

RESULTS: The phenotypic profile and genetic markers of antibiotic resistance of foodborne microorganisms isolated in the Kyrgyz Republic were carried out for the first time. The studies indicate the prevalence of antibiotic-resistant strains in meat and dairy products and water. Each species under investigation belonged to 3 sequences-types: S.aureus (ST5, ST15, ST45), E.faecalis (ST21, ST133, ST179). According to the data obtained, all S.aureus carrying blaZ gene were phenotypically resistant to this group of antibiotics. S. aureus isolates resistant to vancomycin, linezolid and daptomycin did not have genetic determinants of resistance to these antimicrobial agents of last reserve. E.faecalis isolates carrying the tetM gene were phenotypically resistant to tetracycline, with the total number of resistant strains up to 83.3%. The presence of macrolide resistance gene lsaA in E.faecalis investigated is in a good accordance with the expected resistance phenotype of this microorganism.

CONCLUSION: Studies conducted in the Kyrgyz Republic confirm the need to monitor the spread of AMR pathogens through the food chain.

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About the authors

Irina B. Koroleva

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Author for correspondence.
Email: martiusheva@cmd.su
ORCID iD: 0000-0002-9397-9646
SPIN-code: 5463-6656
ResearcherId: LJL-0031-2024

junior researcher of Science group of Antimicrobial resistance of food pathogens

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Nina G. Kulikova

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: kulikova_ng@cmd.su
ORCID iD: 0000-0002-1716-6969
SPIN-code: 8876-0698

PhD (Biol.), senior Researcher of Science group of Antimicrobial resistance of food pathogens

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Lyutsiya A. Bitumina

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: bitumina@cmd.su
ORCID iD: 0000-0002-5378-0827
SPIN-code: 2311-2279
Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Yulia V. Mikhailova

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: mihailova@cmd.su
ORCID iD: 0000-0002-5646-538X
SPIN-code: 4271-1072

Ph.D. in Biology, Head of the Laboratory of Molecular Mechanisms of Antibiotic Resistance

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Andrey A. Shelenkov

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: shelenkov@cmd.su
ORCID iD: 0000-0002-7409-077X
SPIN-code: 6710-7264

PhD (Appl.Math.), senior Researcher of Laboratory of Molecular Mechanisms of Antibiotic Resistance

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Anna E. Karpenko

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: a.egorova@cmd.su
ORCID iD: 0000-0003-0486-1353
SPIN-code: 6350-1373

Researcher of Laboratory of Molecular Mechanisms of Antibiotic Resistance

111123, Russia, Moscow, Novogireevskay st. 3A

Darya K. Kondratieva

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: kondrateva@cmd.su
ORCID iD: 0009-0009-6693-3990
SPIN-code: 4634-9319

junior researcher Laboratory of molecular mechanisms of antibiotic resistance

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

G. E. Amankulova

Department of Disease Prevention and State Sanitary and Epidemiological Surveillance of the Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzstan

Email: amankulova_63@mail.ru

Head of the Reference Laboratory for Antimicrobial Resistance

Kyrgyzstan

A. B. Dzhumakanova

Department of Disease Prevention and State Sanitary and Epidemiological Surveillance of the Ministry of Health of the Kyrgyz Republic, Bishkek, Kyrgyzstan

Email: aigul.dzumakanova.dgsn@mail.ru
ORCID iD: 0009-0005-9065-6744

Head of the Laboratory Testing Center

Kyrgyzstan

Igor N. Manzeniuk

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: manzeniuk@cmd.su
ORCID iD: 0000-0002-1146-1430
SPIN-code: 5013-6441

PhD (Med.), assistant director for research

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

Vasiliy G. Akimkin

FBIS Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Email: vgakimkin@yandex.ru
ORCID iD: 0000-0003-4228-9044
SPIN-code: 4038-7455

Professor, Academician of the Russian Academy of Sciences, MD, Director

Russian Federation, 111123, Russia, Moscow, Novogireevskay st. 3A

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