FEATURES OF THE ETIOLOGY OF PNEUMONIA ASSOCIATED WITH THE SARS-COV-2 VIRUS IN THE PRIMORSKY TERRITORY



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Abstract

 

AIM: comparative analysis of the etiological structure of pneumonia in patients with coronavirus infection caused by SARS-CoV-2 in the Primorsky Territory and assessment of antibiotic resistance of microflora.

METHODS: A retrospective cohort study was conducted of 6491 medical records of patients with confirmed coronavirus infection who were hospitalized between 2021 and 2023. Bacteriological analysis of the biomaterial was performed using differential diagnostic media and identification of isolated pathogens with determination of antibiotic resistance using the BD Phoenix™ automated bacteriological analyzer.

RESULTS: In 2021, pneumonias were predominantly of viral etiology, with a microbial agent detected in only 1/3 of cases. Subsequently, a feature of pneumonia in patients with laboratory-confirmed COVID-19 was a high frequency of mixed infections of bacterial etiology, as well as an increase in the proportion of opportunistic flora in the sputum microbiome (unicellular fungi of the genus Candida, Mycoplasma bacteria) with an increase of more than 6 times by 2023. The isolated bacteria were etiologically significant and belong to the ESKAPE group, in most cases they were obtained in monoculture or together with yeast fungi. Until 2022, gram-positive flora prevailed, and in 2023 its ratio with gram-negative was in equal proportions. The number of methicillin-resistant Staphylococcus aureus reached 52.7%, and resistant to most antibacterial drugs - 77.2%. Gram-positive microorganisms were resistant to penicillin and fluoroquinolone antibiotics, and gram-negative ones were resistant to third-generation cephalosporins, fluoroquinolones and carbapenems.

CONCLUSION: In 2021, pneumonia in sputum was mainly Gram-positive flora, while by 2023 there was an increase in the number of Gram-negative, as well as an increase in opportunistic flora. Antibiotic resistance of the most common gram-positive microorganisms is most pronounced in relation to drugs related to penicillins and fluoroquinolones, and in gram-negative ones to 3rd generation cephalosporins, fluoroquinolones and carbopenems. Thus, the sputum microbiome in pneumonia in patients with coronavirus infection in the Primorsky Territory has its own characteristics, which should be taken into account when prescribing etiotropic therapy.

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About the authors

Svetlana A. Sokotun

Federal State Budgetary Institution of Higher Education "Pacific State Medical University" of the Ministry of Health of the Russian Federation, Vladivostok, Russia

Author for correspondence.
Email: sokotun.s@mail.ru
ORCID iD: 0000-0003-3807-3259
SPIN-code: 8744-2166

Ph.D., Associate Professor of the Department of Infectious Diseases

Russian Federation, 690002, Vladivostok, Ostryakova Avenue, 2

Alexander O. Mikhailov

Pacific State Medical University

Email: mao1991@mail.ru
ORCID iD: 0000-0002-2719-3629
SPIN-code: 1469-9086

Ph.D., Associate Professor of the Department of Infectious Diseases

Russian Federation, 2, Ostryakov Avenue, Vladivostok, 690002, Russian Federation

Anna I. Simakova

Pacific State Medical University; Regional Clinical Hospital No. 2

Email: anna-inf@yandex.ru
ORCID iD: 0000-0002-3334-4673
SPIN-code: 3563-7054

MD, Dr. Sci. (Med.), Associate Professor

Russian Federation, 2 Ostryakova Prospekt, 690002 Vladivostok; Vladivostok

Natalya G. Plekhova

Pacific State Medical University

Email: pl_nat@hotmail.com
ORCID iD: 0000-0002-8701-7213
SPIN-code: 2685-9578

Dr. Sci. (Biology), Assistant Professor

Russian Federation, Vladivostok

Svetlana N. Beniova

Regional Clinical Hospital No. 2

Email: snbeniova@mail.ru
ORCID iD: 0000-0002-8099-1267
SPIN-code: 9715-7742

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Vladivostok

Elena P. Chirkova

State budgetary healthcare institution “Regional Clinical Hospital No. 2

Email: chirkova_ep@kkb2.ru

Deputy Chief Physician for Epidemiological Issues

Russian Federation, 690105, Vladivostok, Russkaya str., 55

Andrey A. Savinov

State budgetary healthcare institution “Regional Clinical Hospital No. 2

Email: savinov.andrey28rus@mail.ru

epidemiologist

690105, Vladivostok, Russkaya str., 55

References

  1. Avdeeva M. G. et al. Microbial landscape in hospital patients with a new coronavirus infection COVID-19, comparative antibiotic resistance with the "pre-covid" period: a prospective study. – 2021. – T. 28. – №. 5. – P. 14-28.
  2. Krivoruchko I. V., Kashirina L. A., Pritulina Y. G. Microbiological study of sputum microflora of patients with community-acquired pneumonia caused by the SARS-COV-2 virus. – 2021. – T. 26. – №. 4. – P. 20-24.
  3. Tsyrkunov V. M., Potapchik A. L., Girgel A. N. Microbial landscape in patients of the department of resuscitation and anesthesiology during the COVID-19 pandemic. – 2022. – T. 35. – №. 1. – P. 64-70.
  4. Zaitsev A. A. et al. Prakticheskii opyt vedeniya bol'tov s novoy koronavnoy infektsiyu COVID-19 v instorinate (predstavlye itogi i rekomendatsii) [Practical experience in the management of patients with a new coronavirus infection COVID-19 in a hospital (preliminary results and recommendations)]. – 2020. – №. 6. – P. 74-79.
  5. Kataeva L. V., et al. Microbiocenosis of the lower respiratory tract in pneumonia associated with SARS-CoV-2. – 2020.
  6. Temporary Guidelines "Prevention, Diagnosis and Treatment of a New Coronavirus Infection (COVID-19)". Version 10 (08.02.2021).
  7. Romashov O. M., et al. Assessment of the resistance of microorganisms of a multidisciplinary hospital and modernization of antimicrobial therapy schemes in the context of the COVID-19 pandemic. – 2021. – T. 23. – №. 3. – P. 293-303.
  8. Eidelshtein I.A. Mycoplasma pneumoniae – modern data on the structure, molecular biology and epidemiology of the pathogen // Clinical microbiology and antimicrobial therapy. - 2023. - №4. - T.25. - P.332-350.
  9. Mirzaei R. et al. Bacterial co‐infections with SARS‐CoV‐2 //IUBMB life. – 2020. – Т. 72. – №. 10. – С. 2097-2111.
  10. Sharifipour E. et al. Evaluation of bacterial co-infections of the respiratory tract in COVID-19 patients admitted to ICU //BMC infectious diseases. – 2020. – Т. 20. – С. 1-7.

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