Email this article Sensitivity of pathogenic and opportunistic microorganisms to probiotic strains in the light of personalized probiotic therapy
- Authors: Parakhina A.I.1, Parakhina L.I.1, Troshina D.A.2, Levina K.E.2, Pimenova Y.A.1, Koteneva E.N.1, Evstropov A.N.1, Zakharova L.N.1, Mironov A.Y.3,4, Suranova T.G.4
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Affiliations:
- Novosibirsk State Medical University
- Center of Hygiene and Epidemiology in the Novosibirsk Region
- Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky
- Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
- Issue: Vol 27, No 5 (2022)
- Pages: 280-289
- Section: ORIGINAL STUDIES
- URL: https://rjeid.com/1560-9529/article/view/117545
- DOI: https://doi.org/10.17816/EID117545
- ID: 117545
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Abstract
BACKGROUND: Resistance of microorganisms to antimicrobial drugs (AMP) is one of the global problems of modern medicine, which poses a serious threat to human health. The formation of the resistance of pathogens to AMP forces us to look for new ways to combat them. Probiotic therapy is one of the promising and safe methods of treating many infectious diseases.
AIM: This study assesses the sensitivity of clinical strains of pathogenic and opportunistic bacteria and fungi to microorganisms that are part of probiotic preparations.
MATERIALS AND METHODS: The sensitivity of 93 cultures of pathogenic and opportunistic microorganisms (UPM) isolated from the feces of patients with intestinal dysbiosis to strains of probiotic bacteria isolated from commercial probiotic preparations was studied: Lactobacillus plantarum 8RA-3, Escherichia coli M-17, Bacillus subtilis strain VKPM B-10641, Enterococcus faecium SF-68, Saccharomyces boulardii CNCM I-745 by the delayed antagonism method.
RESULTS: Pathogenic microorganisms showed low and moderate sensitivity to the probiotic strain of L. plantarum. Sensitivity to the probiotic strain of B. subtilis was detected in Staphylococcus aureus strains, low or completely absent in Pseudomonas aeruginosa, Klebsiella spp., Serratia marcescens. The most sensitive to the probiotic strain were clinical strains of E. coli.
CONCLUSION: UPM and pathogenic microorganisms isolated from the gastrointestinal tract of patients with dysbiosis have different sensitivity to probiotic strains: from high to several probiotics to its complete absence. The empirical approach to prescribing a probiotic does not guarantee a therapeutic effect, which makes it prudent to individualize the selection of a probiotic based on an assessment of its antimicrobial activity against isolated pathogens.
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About the authors
Anastasia I. Parakhina
Novosibirsk State Medical University
Email: an.parakhina@mail.ru
ORCID iD: 0000-0003-1108-0811
Russian Federation, Novosibirsk
Larisa I. Parakhina
Novosibirsk State Medical University
Email: myinspirationmy@mail.ru
ORCID iD: 0000-0003-2428-9032
Russian Federation, Novosibirsk
Darya A. Troshina
Center of Hygiene and Epidemiology in the Novosibirsk Region
Email: troshina.darya92@mail.ru
ORCID iD: 0000-0003-3658-2674
Russian Federation, Novosibirsk
Katerina E. Levina
Center of Hygiene and Epidemiology in the Novosibirsk Region
Email: pumpum235@gmail.ru
ORCID iD: 0000-0001-6550-2931
Russian Federation, Novosibirsk
Yulia A. Pimenova
Novosibirsk State Medical University
Email: medic.81@mail.ru
ORCID iD: 0000-0001-8722-2746
SPIN-code: 6979-3750
MD, Cand. Sci. (Med.), Associate Professor
Russian Federation, NovosibirskElena N. Koteneva
Novosibirsk State Medical University
Email: lenakoteneva@rambler.ru
ORCID iD: 0000-0002-9901-2292
SPIN-code: 6638-0005
MD
Russian Federation, NovosibirskAleksandr N. Evstropov
Novosibirsk State Medical University
Email: evstrop@gmail.com
ORCID iD: 0000-0002-1551-3154
SPIN-code: 1747-5483
MD, Dr. Sci. (Med.), Professor
Russian Federation, NovosibirskLiudmila N. Zakharova
Novosibirsk State Medical University
Email: nisngma@ngs.ru
ORCID iD: 0000-0001-6051-2380
SPIN-code: 8610-8837
MD, Cand. Sci. (Med.), Associate Professor
Russian Federation, NovosibirskAndrey Yu. Mironov
Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky; Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
Email: andy.60@mail.ru
ORCID iD: 0000-0002-8544-5230
SPIN-code: 9225-1560
MD, Dr. Sci. (Med.), Professor
Russian Federation, Moscow; MoscowTatyana G. Suranova
Academy of Postgraduate Education of the Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
Author for correspondence.
Email: suranovatatiana@mail.ru
ORCID iD: 0000-0003-3411-1027
SPIN-code: 7326-5273
MD, Cand. Sci. (Med.), Associate Professor
Russian Federation, MoscowReferences
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