PCR IDENTIFICATION OF FRAGMENTS OF GENES FOR PATHOGENICITY ISLANDS IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS



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Abstract

The identification of fragments of pathogenicity islands (SAPI1, vSaα and vSaβ) in 11 clinical strains of Methicillin-resistant Staphylococcus aureus (MRSA), referred on the basis of spa- sequence typing and the restriction-modification test to different genotypes, has been performed. A set of studied pathogenicity islands was shown to be strain-specific and be available for usage as an epidemiological marker of hospital strains of Staphylococcus aureus.

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ПЦР-ИДЕНТИФИКАЦИЯ ФРАГМЕНТОВ ГЕНОВ ОСТРОВОВ ПАТОГЕННОСТИ В МЕТИЦИЛЛИНРЕЗИСТЕНТНЫХ ШТАММАХ STAPHYLOCOCCUS AUREUS
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About the authors

A. E Goncharov

Federal State Budgetary Institution of Higher Professional Education"The St. Petersburg 1.1. Mechnikov State Medical Academy"

Email: phage1@yandex.ru
47, Piskarevskiy prospekt, St. Petersburg, Russian Federation, 195067

L. P Zueva

Federal State Budgetary Institution of Higher Professional Education"The St. Petersburg 1.1. Mechnikov State Medical Academy"

Email: uzueva@mail.ru
47, Piskarevskiy prospekt, St. Petersburg, Russian Federation, 195067

A. E. Suvorov

State Budgetary Institution"Institute of Experimental Medicine of the NorthWest Branch of the Russian Academy of Medical Sciences"

Email: suvorov1@hotmail.com
12, Akad.Pavlov Str., St.Petersburg, Russian Federation, 197376

L. S Glazovskaya

State Budgetary Educational Institution of Higher Professional Education"Kemerovo state medical academy" of the Ministry of Health care

22a, Voroshilova Str., Kemerovo, 650029

E. B Brusina

State Budgetary Educational Institution of Higher Professional Education"Kemerovo state medical academy" of the Ministry of Health care

Email: brusina@mail.ru
22a, Voroshilova Str., Kemerovo, 650029

I. S. Azizov

The Republican State Enterprise"Karaganda State Medical University"

Email: antibiotic@mail.kz
40, Gogolya Str., Karaganda, The Republic of Kazakhstan, 100008

A. V Lavrinenko

The Republican State Enterprise"Karaganda State Medical University"

Email: lavrinenko.alena@gmail.com
40, Gogolya Str., Karaganda, The Republic of Kazakhstan, 100008

T. N Suborova

State Budgetary Educational Institution of Higher Professional Education"Kemerovo state medical academy" of the Ministry of Health care

22a, Voroshilova Str., Kemerovo, 650029

D. V Razumova

The Federal State Treasury Military Educational Institution of Higher Professional Education"Military-medical Academy named after S. M. Kirov" of the Ministry of Defense

6-Zh, Akademika Lebedeva Str., St.-Petersburg, Russian Federation, 194044

B. I Aslanov

Federal State Budgetary Institution of Higher Professional Education"The St. Petersburg 1.1. Mechnikov State Medical Academy"

Email: khoroshiloff@rambler.ru
47, Piskarevskiy prospekt, St. Petersburg, Russian Federation, 195067

A. A Dolgiy

Federal State Budgetary Institution of Higher Professional Education"The St. Petersburg 1.1. Mechnikov State Medical Academy"

Email: batyra@mail.ru
47, Piskarevskiy prospekt, St. Petersburg, Russian Federation, 195067

A. A Dolgiy

Federal State Budgetary Institution of Higher Professional Education"The St. Petersburg 1.1. Mechnikov State Medical Academy"

Email: zoy4@yandex.ru
47, Piskarevskiy prospekt, St. Petersburg, Russian Federation, 195067

References

  1. Афанасьев М.В., Каракашев С.В., Ильина Е.Н., Салем А.-С.-А.-М., Сидоренко С.В., Говорун В.М. Молекулярно-генетическая характеристика метициллинустойчивых штаммов Staphylococcus aureus, выделенных в стационарах г. Москвы. Молекулярная генетика, микробиология и вирусология. 2010; 2: 20-4.
  2. Гончаров А.Е., Olsson-Liljequist B., Зуева Л.П. и др. Эпидемический штамм метициллинрезистентного Staphylococcus aureus в стационарах Санкт-Петербурга. Журнал микробиологии, эпидемиологии и иммунобиологии. 2010; 5: 24-9.
  3. Дмитренко О.А., Шагинян И.А., Прохоров В.Я., Матвеев С.М., Гинцбург А.Л. Молекулярно-генетическое типирование метициллинрезистентных штаммов Staphylococcus aureus, выделенных в разных регионах Российской Федерации, на основании изучения размеров продукта амплификации и последующего рестрикционного анализа коагулазного гена. Журнал микробиологии, эпидемиологии и иммунобиологии. 2005; 4: 46-52
  4. Cockfield J.D., Pathak S., Edgeworth J.D., Lindsay J.A. Rapid determination of hospital-acquired meticillin-resistant Staphylococcus aureus lineages. J. Med. Microbiol. 2007; 56(Pt 5): 614-9.
  5. Emori T.G., Gaynes R.P. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin. Microbiol. Rev. 1993; 6(4): 428-42.
  6. Harmsen D., Claus H., Witte W. et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 2003; 41: 5442-8.
  7. Kuroda M. et al. Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet. 2001;.357: 1225-40.
  8. Malachowa N., DeLeo F.R. Mobile genetic elements of Staphylococcus aureus. Cell. Mol. Life Sci. 2010; 67: 3057-71.
  9. Tendolkar P.M., Baghdayan A.S., Shankar N. Pathogenic enterococci: new developments in the 21-st century. Cell. Mol. Life Sci. 2003; 60: 3-15.

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