Associations Oxidative Stress Peripheral Markers with Clinical Characteristics and Inflammatory Factors in Alcoholic Patients

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

A fundamental problem in narcology is to clarify the mechanisms of development of alcohol dependence (AD), in the pathogenesis of which disturbances in redox processes and inflammation play an important role. Identification of associations between biological and clinical parameters clarifies the molecular mechanisms of disease pathogenesis. The aim of the work was to study peripheral markers of oxidative stress (OS) in patients with AD in the early period of post-abstinence state and to identify their relationship with the clinical characteristics of the disease and inflammatory factors. The parameters of 84 male AD patients were determined; the average age was 44.3 ± 8.2 years. Clinical characteristics were analyzed: patient age, age of alcohol withdrawal syndrome (AWS) formation, duration of the disease, duration of AWS. OS markers were determined in blood plasma – protein oxidation products (carbonyl proteins, CP) using 2,4-dinitrophenylhydrazine; lipids (products reacting with thiobarbituric acid (TBA), TBA-RP); DNA (8-hydroxy-2′-deoxyguanosine, 8-OH-dG). Inflammatory mediators – proinflammatory cytokines (IFNγ, IL-1β, IL-6, IL-8, IL-17A, TNFα) were determined in blood serum. Control for biological studies – blood samples of 80 conditionally healthy men, average age 40.9 ± 9.6 years. In patients with AD, an increase in CP, TBA-RP and all cytokines was detected compared to controls (p < 0.001); the concentration of 8-OH-dG did not differ from the control. The following were found: a direct connection between TBA-RP and the duration of the disease; inverse relationship between CP and the age of formation and duration of AWS. CP had an inverse relationship with IL-6. Positive correlations were found between 8-OH-dG and IL-6, TBA-RP and IL-8, TBA-RP and TNFα. Thus, the early period of the post-withdrawal state in AD patients is characterized by pronounced OS and inflammation. The results obtained expand knowledge about the integrative contribution of OS and inflammation factors to the pathogenesis of AD and can be used in the development of new treatment methods.

Full Text

Restricted Access

About the authors

V. D. Prokopieva

Tomsk National Research Medical Center of the Russian Academy of Sciences

Author for correspondence.
Email: valyaprok@mail.ru
Russian Federation, 634014, Tomsk

T. P. Vetlugina

Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: valyaprok@mail.ru
Russian Federation, 634014, Tomsk

E. V. Epimakhova

Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: valyaprok@mail.ru
Russian Federation, 634014, Tomsk

A. S. Boyko

Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: valyaprok@mail.ru
Russian Federation, 634014, Tomsk

N. A. Bokhan

Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk State University

Email: valyaprok@mail.ru
Russian Federation, 634014, Tomsk; 634050, Tomsk

References

  1. Zima, T., Albano, E., Ingelman-Sundberg, M., Arteel, G. E., Thiele, G. M., Klassen, L. W., and Sun A. Y. (2005) Modulation of oxidative stress by alcohol, Alcoholism, 29, 1060-1065, https://doi.org/10.1097/01.ALC. 0000168168.43419.54
  2. Huang, M. C., Chen, C. H., Peng, F. C., Tang, S. H., and Chen C. C. (2009) Alterations in oxidative stress status during early alcohol withdrawal in alcoholic patients, J. Formos. Med. Assoc., 108, 560-569, https://doi.org/10.1016/S0929-6646(09)60374-0.
  3. Прокопьева В. Д., Ветлугина Т. П. (2023) Особенности окислительного стресса при алкоголизме (обзор), Биомед. Хим., 69, 83-96, https://doi.org/10.18097/PBMC20236902083.
  4. Coleman, L. G. Jr., and Crews, F. T. (2018) Innate immune signaling and alcohol use disorders, Handb. Exp. Pharmacol., 248, 369-396, https://doi.org/10.1007/164_2018_92.
  5. Crews, F. T., Lawrimore, C. J., Walter, T. J., and Coleman, L. G. Jr. (2017) The role of neuroimmune signaling in alcoholism, Neuropharmacology, 122, 56-73, https://doi.org/10.1016/j.neuropharm.2017.01.031.
  6. Airapetov, M., Eresko, S., Lebedev, A., Bychkov, E., and Shabanov, P. (2021) The role of Toll-like receptors in neurobiology of alcoholism, Biosci. Trends., 15, 74-82, https://doi.org/10.5582/bst.2021.01041.
  7. Erickson, E. K., Grantham, E. K., Warden, A. S., and Harris, R. A. (2019) Neuroimmune signaling in alcohol use disorder, Pharmacol. Biochem. Behav., 177, 34-60, https://doi.org/10.1016/j.pbb.2018.12.007.
  8. Agita, A., and Alsagaff, M. T. (2017) Inflammation, immunity, and hypertension, Acta Med. Indone, 49, 158-165.
  9. Yan, C., Hu, W., Tu, J., Li, J., Liang, Q., and Han, S. (2023) Pathogenic mechanisms and regulatory factors involved in alcoholic liver disease, J. Transl. Med., 21, 300, https://doi.org/10.1186/s12967-023-04166-8.
  10. Ветлугина Т. П., Прокопьева В. Д., Бохан Н. А. (2023) Биологические основы адъювантной терапии алкоголизма, Томск, Изд-во Томского государственного университета, 208 с.
  11. Levine, R. L. (2002) Carbonyl modified proteins in cellular regulation, aging and disease, Free Radic. Biol. Med., 32, 790-796.
  12. Yildirim, T., Armutcu, F., Hasgul, R., Yuksel, R. N., Sengezer, T., and Erdamar, H. (2013) Oxidative stress markers, alpha-fetoprotein and alpha-fetoprotein-L3 levels of alcohol dependent subjects, Oxid. Antioxid. Med. Sci., 2, 303-307, https://doi.org/10.5455/oams.031013.or.053.
  13. Galicia-Moreno, M., Rosique-Oramas, D., Medina-Avila, Z., Álvarez-Torres, T., Falcón, D., Higuera-de la Tijera, F., Béjar, Y. L., Cordero-Pérez, P., Muñoz-Espinosa, L., Pérez-hernández, J. L., Kershenobich, D., and Gutierrez-Reyes, G. (2016) Behavior of oxidative stress markers in alcoholic liver cirrhosis patients, Oxid. Med. Cell Longev., 9370565, https://doi.org/10.1155/2016/9370565.
  14. Прокопьева В. Д., Ярыгина Е. Г., Плотников Е. В., Ветлугина Т. П. (2019) Исследование эффектов солей лития в присутствии этанола на продукт окислительного повреждения ДНК плазмы крови здоровых лиц и больных алкоголизмом, Сиб. Вестн. Психиатр. Наркол., 1, 5-11, https://doi.org/10.26617/1810-3111-2019-1(102)-5-11.
  15. Chen, C. H., Pan, C. H., Chen, C. C., and Huang, M. C. (2011) Increase oxidative DNA damage in patients with alcohol dependence and its correlation with alcohol withdrawal severity, Alcohol Clin. Exp. Res., 35, 338-344, https://doi.org/10.1111/j.1530-0277.2010.01349.
  16. Черников А. В., Гудков С. В., Усачева А. М., Брусков В. И. (2017) Экзогенный 8-оксо-7,8-дигидро-2′-дезоксигуанозин: биомедицинские свойства, механизмы действия, терапевтический потенциал, Усп. Биол. Хим., 57, 267-302, https://doi.org/10.1134/S0006297917130089.
  17. Cooke, M. S., Evans, M. D., Dizdaroglu, M., and Lunec, J. (2003) Oxidative DNA damage: mechanisms, mutation, and disease, FASEB J., 17, 1195-1214.
  18. Kasai, H. (1997) Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis, Mutat. Res., 387, 147-163.
  19. Мармий Н. В., Есипов Д. С. (2015) Биологическая роль 8-оксо-2′-дезоксигуанозина, Вестник Моск. Универ. Сер. Биол., 4, 19-23.
  20. Ветлугина Т. П., Никитина В. Б., Мандель А. И., Бойко А. С., Прокопьева В. Д., Бохан Н. А. (2019) Факторы иммуноэндокринной регуляции при алкогольной зависимости на этапе формирования терапевтической ремиссии, Росс. Иммунол. Журн., 13, 183-186, https://doi.org/10.31857/S102872210006447-6.
  21. Ветлугина Т. П., Прокопьева В. Д., Епимахова Е. В., Бойко А. С., Никитина В. Б., Бохан Н. А. (2022) Продукция цитокинов в культуре клеток крови больных алкогольной зависимостью и маркеры окислительного стресса аутологичной плазмы, Клет. Технол. Биол. Мед., 1, 45-48, https://doi.org/10.47056/1814-34902022-1-45-48.
  22. Dong, D., Zhong, W., Sun, Q., Zhang, W., Sun, X., and Zhou, Z. (2016) Oxidative products from alcohol metabolism differentially modulate pro-inflammatory cytokine expression in Kupffer cells and hepatocytes, Cytokine, 85, 109-119, https://doi.org/10.1016/j.cyto.2016.06.014.
  23. Jacob, K. D., Noren Hooten, N., Trzeciak, A. R., and Evans, M. K. (2013) Markers of oxidant stress that are clinically relevant in aging and age-related disease, Mech. Ageing Dev., 134, 139-157, https://doi.org/10.1016/ j.mad.2013.02.008.
  24. Gladyshev, V. N., Kritchevsky, S. B., Clarke, S. G., Cuervo, A. M., Fiehn, O., de Magalhães, J. P., Mau, T., Maes, M., Moritz, R., Niedernhofer, L. J., Van Schaftingen, E., Tranah, G. J., Walsh, K., Yura, Y., Zhang, B., and Cummings, S. R. (2021) Molecular damage in aging, Nat. Aging, 1, 1096-1106, https://doi.org/10.1038/s43587021-00150-3.
  25. Spiteller, G. (2001) Lipid peroxidation in aging and age-dependent diseases, Exp. Gerontol., 36, 1425-1457, https://doi.org/10.1016/s0531-5565(01)00131-0.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Russian Academy of Sciences