Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead


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Abstract

Background:Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite.

Objective:To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis.

Methods:The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH.

Results:In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs’ clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity.

Conclusion:The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.

About the authors

Lais Nogueira

Laboratório de Biotecnologia de Microrganismos,, Federal University of São João del-Rei

Email: info@benthamscience.net

Juliana Machado

Laboratório de Biotecnologia de Microrganismos,, Federal University of São João del-Rei

Email: info@benthamscience.net

Daniel Dias

Laboratório de Biotecnologia de Microrganismos, Federal University of São João del-Rei

Email: info@benthamscience.net

Rita Rodrigues

Laboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei

Email: info@benthamscience.net

Mariana da Paz

Laboratório de Nanobiotecnologia & Bioativos, Universidade Federal de São João Del-Rei

Email: info@benthamscience.net

Rafael Teixeira-Neto

Laboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei

Email: info@benthamscience.net

Alexandre Reis

Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB),, Universidade Federal de Ouro Preto

Email: info@benthamscience.net

Alexsandro Galdino

Laboratório de Biotecnologia de Microrganismos, Federal University of São João del-Rei

Author for correspondence.
Email: info@benthamscience.net

Eduardo Silva

Laboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei

Author for correspondence.
Email: info@benthamscience.net

Henrique Moura

Laboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-Rei

Email: info@benthamscience.net

Henrique Ker

Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto

Email: info@benthamscience.net

Luana Ramos

Laboratório de Biotecnologia de Microrganismos, Federal University of São João del-Rei

Email: info@benthamscience.net

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