Evaluation of the Antioxidant and Anti-inflammatory Activity of the Anacyclus Valentinus L. Essential Oil and its Oxygenated Fraction


Дәйексөз келтіру

Толық мәтін

Аннотация

Aim and Objective:Synthetic antioxidants and anti-inflammatories are widely used by the food and pharmaceutical industries. Like any synthetic product, these are toxic and represent a significant health risk. The objective of this study was to determine the chemical composition of Anacyclus valentinus essential oil and its oxygenated fraction, as well as their in-vitro antioxidant and anti-inflammatory properties.

Methods:The essential oil was hydrodistilled using a Clevenger-type device and the oxygenated fraction was obtained by column chromatography using diethyl ether. The essential oil and its oxygenated fraction were analysed by GC and GC/MS. The antioxidant activities were performed using three different methods Radical scavenging activity (DPPH), β-carotene bleaching test, and the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. The activity of antiinflammatory of essential oil and its oxygenated fraction was assessed by the protein denaturation method using the diclofenac sodium as a positive control.

Results:The essential oil of Anacyclus valentinus was represented mainly by oxygenated sesquiterpene compounds (37.7%), hydrocarbon sesquiterpenes (14.7%), oxygenated monoterpenes (18.4%) and non-terpenic compounds (15.6%). The oxygenated fraction was composed mainly of oxygenated sesquiterpenes (40.6%), oxygenated monoterpenes (38.5%), and non-terpene compounds (19.4%). Essential oil and oxygenated fraction showed antioxidant activity. The most potent activity was observed by the oxygenated fraction in the DPPH (IC50 = 8.2 mL/L) and β- carotene bleaching (IC50 = 5.6 mL/L) tests. While the essential oil of A. valentinus showed very good anti-inflammatory activity with an IC50 of 0.3 g/L higher than that of diclofenac (IC50= 0.53 g/L).

Conclusion:The results showed that the essential oil and the oxygenated fraction of A. valentinus are rich in sesquiterpene compounds and possess interesting antioxidant and anti-inflammatory properties. However, other studies are necessary to be able to offer these extracts to the pharmaceutical and food industries.

Авторлар туралы

Nabila Ainseba

Laboratoire de Chimie Organique, Substances Naturelles et Analyses (COSNA, Université de Tlemcen,

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

Amina Soulimane

Laboratoire d’Ecologie et Gestion des Ecosystèmes Naturels (LECGEN), Université de Tlemcen

Email: info@benthamscience.net

Imane Mami

Laboratoire de Chimie Organique, Substances Naturelles et Analyses (COSNA),, Université de Tlemcen

Email: info@benthamscience.net

Mohammed Dib

Laboratoire des Substances Naturelles et Bioactives (LASNABIO),, Université de Tlemcen

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

Alain Muselli

Laboratoire Chimie des Produits Naturels, Université de Corse,

Email: info@benthamscience.net

Әдебиет тізімі

  1. Gogoi, R.; Loying, R.; Sarma, N.; Munda, S.; Kumar Pandey, S.; Lal, M. A comparative study on antioxidant, anti-inflammatory, genotoxicity, anti-microbial activities and chemical composition of fruit and leaf essential oils of Litsea cubeba Pers from North-east India. Ind. Crops Prod., 2018, 125, 131-139. doi: 10.1016/j.indcrop.2018.08.052
  2. Rao, M.R.; Palada, M.C.; Becker, B.N. Medicinal and aromatic plants in agroforestry systems. Agrofor. Syst., 2004, 61-62(1-3), 107-122. doi: 10.1023/B:AGFO.0000028993.83007.4b
  3. Sivropoulou, A.; Papanikolaou, E.; Nikolaou, C.; Kokkini, S.; Lanaras, T.; Arsenakis, M. Antimicrobial and cytotoxic activities of origanum essential oils. J. Agric. Food Chem., 1996, 44(5), 1202-1205. doi: 10.1021/jf950540t
  4. Ainane, T.; Abdoul-Latif, F.M.; El Montassir, Z.; Attahar, W.; Ainane, A.; Giuffrè, A.M. Essential oils rich in pulegone for insecticide purpose against legume bruchus species: Case of Ziziphora hispanica L. and Mentha pulegium L. AIMS Agric. Food, 2022, 8(1), 105-118.
  5. Ebrahimi, S.; Paryad, E.; Ghanbari Khanghah, A.; Pasdaran, A.; Kazemnezhad Leili, E.; Sadeghi Meibodi, A.M. The effects of lavandula aromatherapy on pain relief after coronary artery bypass graft surgery: A randomized clinical trial. Appl. Nurs. Res., 2022, 68, 151638. doi: 10.1016/j.apnr.2022.151638 PMID: 36473717
  6. McDonnell, B.; Newcomb, P. Trial of essential oils to improve sleep for patients in cardiac rehabilitation. J. Altern. Complement. Med., 2019, 25(12), 1193-1199. doi: 10.1089/acm.2019.0222 PMID: 31556690
  7. Mele, M.A. Bioactive compounds and biological activity of ginger. J. Multidiscip. Sci., 2019, 1(1), 1-7. doi: 10.33888/jms.2019.111
  8. Gioffrè, G.; Ursino, D.; Labate, M.L.C.; Giuffrè, A.M. The peel essential oil composition of bergamot fruit (Citrus bergamia, Risso) of Reggio Calabria (Italy): A review. Emir. J. Food Agric., 2020, 32(11), 835-845. doi: 10.9755/ejfa.2020.v32.i11.2197
  9. Giuffrè, A.M. Citrus bergamia, Risso: The peel, the juice and the seed oil of the bergamot fruit of Reggio Calabria. Emir. J. Food Agric., 2020, 32(7), 522-532.
  10. Şanli, A.; Karadoğan, T. Geographical impact on essential oil composition of endemic Kundmanniaanatolica HUB.-MOR.(APIACEAE). Afr. J. Tradit. Complement. Altern. Med., 2016, 14(1), 131-137. doi: 10.21010/ajtcam.v14i1.14 PMID: 28480390
  11. Chatterjee, S.; Gupta, S.; Variyar, P.S. Comparison of Essential oils obtained from different extraction techniques as an aid in identifying aroma significant compounds of nutmeg (Myristica Fragrans). Nat. Prod. Commun., 2015, 10(8), 1934578X1501000. doi: 10.1177/1934578X1501000833 PMID: 26434138
  12. Ložienė K.; Venskutonis, P.R. Influence of environmental and genetic factors on the stability of essential oil composition of Thymus pulegioides. Biochem. Syst. Ecol., 2005, 33(5), 517-525. doi: 10.1016/j.bse.2004.10.004
  13. Bremer, K.; Humphries, C. Generic monograph of the Asteraceae-Anthemidae. Bull. Nat. Hist. Mus, 1993, 23(2), 71-177.
  14. Side Larbi, K.; Meddah, B.; Tir Touil Meddah, A.; Sonnet, P. The antibacterial effect of two medicinal plants Inula viscosa, Anacyclus valentinus (Asteraceae) and their synergistic interaction with antibiotic drugs. Rev. Sci. Fondam. Appl., 2016, 8(2), 244-255. doi: 10.4314/jfas.v8i2.5
  15. Greger, H. Comparative phytochemistry and systematics of Anacyclus. Biochem. Syst. Ecol., 1978, 6(1), 11-17. doi: 10.1016/0305-1978(78)90019-4
  16. Houicher, A.; Hamdi, M.; Hechachna, H.; Özogul, F. Chemical composition and antifungal activity of Anacyclus valentinus essential oil from Algeria. Food Biosci., 2018, 25, 28-31. doi: 10.1016/j.fbio.2018.07.005
  17. European Pharmacopoeia, 5th ed; Council of Europe: Strasbourg, 2004, pp. 217-218.
  18. Dib, M.E.A.; Benhamidat, L.; Bensaid, O.; Keniche, A. ouar, I.E.; Muselli, A. Inflammatory and neuroprotective properties of hexane extracts from the roots of Centaurea acaulis and Centaurea pullata. Antiinfect. Agents, 2022, 20(5), e100622205831. doi: 10.2174/2211352520666220610113750
  19. Benhamidat, L.; Amine Dib, M.E.; Bensaid, O.; Zatla, A.T.; Keniche, A.; Ouar, I.E.; Nassim, D.; Muselli, A. chemical composition and antioxidant, anti-inflammatory and anticholinesterase properties of the aerial and root parts of Centaurea acaulis essential oils: Study of the combinatorial activities of aplotaxene with reference standards. J. Essent. Oil-Bear. Plants, 2022, 25(1), 126-146. doi: 10.1080/0972060X.2022.2046177
  20. Mami, I.R.; Merad-Boussalah, N.; El Amine Dib, M.; Tabti, B.; Costa, J.; Muselli, A. Chemical variability and antioxidant activities of the essential oils of the aerial parts of Ammoides verticillata and the roots of Carthamus caeruleus and their synergistic effect in combination. Comb. Chem. High Throughput Screen., 2021, 24(1), 71-78. doi: 10.2174/1386207323666200606213057 PMID: 32504498
  21. Achiri, R.; Benhamidat, L.; Mami, I.R.; Dib, M.E.A.; Aissaoui, N.; Cherif, C.Z.; Cherif, H.Z.; Muselli, A. Chemical composition and antioxidant, anti-inflammatory and antimicrobial activities of the essential oil and its major component (Carlina oxide) of Carlina hispanica roots from western algeria. J. Essent. Oil-Bear. Plants, 2021, 24(5), 1113-1124. doi: 10.1080/0972060X.2021.2005692
  22. Konig, W.; Joulain, D.; Hochmuth, D. Terpenoids and related constituents of essential oils; library of mass finder 2.1; Institute of Organic Chemistry, University of Hamburg, 2001.
  23. Mc Lafferty, F.; Stauffer, D. Wiley register of mass spectral datamass spectrometry library search system bench-Top/PBM; 6th ed Version, 1994.
  24. Mc Lafferty, F.W.; Stauffer, D.B. The Wiley/NBS registry of mass spectra data, 1st ed; Wiley-Interscience: New York, 1988.
  25. Standards, N.I.O. Technology, PC Version 1.7 of The NIST/EPA/NIH Mass Spectral Library. 1999.
  26. Belabbes, R.; Dib, M.E.A.; Djabou, N.; Ilias, F.; Tabti, B.; Costa, J.; Muselli, A. Chemical variability, antioxidant and antifungal activities of essential oils and hydrosol extract of Calendula arvensis L. from Western Algeria. Chem. Biodivers., 2017, 14(5), e1600482. doi: 10.1002/cbdv.201600482 PMID: 28109063
  27. Thapa, P.; Prakash, O.; Rawat, A.; Kumar, R.; Srivastava, R.M.; Rawat, D.S.; Pant, A.K. Essential oil composition, antioxidant, anti-inflammatory, insect antifeedant and sprout suppressant activity in essential oil from aerial parts of Cotinus coggygria scop. J. Essent. Oil-Bear. Plants, 2020, 23(1), 65-76. doi: 10.1080/0972060X.2020.1729246
  28. Bougatef, A.; Hajji, M.; Balti, R.; Lassoued, I.; Triki-Ellouz, Y.; Nasri, M. Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food Chem., 2009, 114(4), 1198-1205. doi: 10.1016/j.foodchem.2008.10.075
  29. Sangita, C.; Priyanka, C.; Protapaditya, D.; Sanjib, B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac. J. Trop. Biomed., 2012, 1, S178-S180.
  30. Telci, I.; Demirtas, I.; Sahin, A. Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Ind. Crops Prod., 2009, 30(1), 126-130. doi: 10.1016/j.indcrop.2009.02.010
  31. Zobayed, S.M.A.; Afreen, F.; Kozai, T. Phytochemical and physiological changes in the leaves of St. John’s wort plants under a water stress condition. Environ. Exp. Bot., 2007, 59(2), 109-116. doi: 10.1016/j.envexpbot.2005.10.002
  32. Dash, S.; Mohanty, N. Evaluation of assays for the analysis of thermo-tolerance and recovery potentials of seedlings of wheat (Triticum aestivum L.) cultivars. J. Plant Physiol., 2001, 158(9), 1153-1165. doi: 10.1078/0176-1617-00243
  33. Ruberto, G.; Baratta, M.T. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem., 2000, 69(2), 167-174. doi: 10.1016/S0308-8146(99)00247-2
  34. Lahlou, M. Methods to study the phytochemistry and bioactivity of essential oils. Phytother. Res., 2004, 18(6), 435-448. doi: 10.1002/ptr.1465 PMID: 15287066
  35. Souza, M.C.; Siani, A.C.; Ramos, M.F.S.; Menezes-de-Lima, O.J.; Henriques, M.G.M.O. Evaluation of anti-inflammatory activity of essential oils from two Asteraceae species. Pharmazie, 2003, 58(8), 582-586. PMID: 12967039
  36. Passos, G.F.; Fernandes, E.S.; da Cunha, F.M.; Ferreira, J.; Pianowski, L.F.; Campos, M.M.; Calixto, J.B. Anti-inflammatory and anti-allergic properties of the essential oil and active compounds from Cordia verbenacea. J. Ethnopharmacol., 2007, 110(2), 323-333. doi: 10.1016/j.jep.2006.09.032 PMID: 17084568
  37. Fernandes, E.S.; Passos, G.F.; Medeiros, R.; da Cunha, F.M.; Ferreira, J.; Campos, M.M.; Pianowski, L.F.; Calixto, J.B. Anti-inflammatory effects of compounds alpha-humulene and (−)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. Eur. J. Pharmacol., 2007, 569(3), 228-236. doi: 10.1016/j.ejphar.2007.04.059 PMID: 17559833
  38. Hart, P.H.; Brand, C.; Carson, C.F.; Riley, T.V.; Prager, R.H.; Finlay-Jones, J.J. Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes. Inflamm. Res., 2000, 49(11), 619-626. doi: 10.1007/s000110050639 PMID: 11131302
  39. Bolzani, V.S.; Valli, M.; Pivatto, M.; Viegas, C. Jr Natural products from Brazilian biodiversity as a source of new models for medicinal chemistry. Pure Appl. Chem., 2012, 84(9), 1837-1846. doi: 10.1351/PAC-CON-12-01-11

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