Qingrexiaoji Recipe Regulates the Differentiation of M2 TAM via miR-29 in GC


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Abstract

Background:Gastric cancer, one of the most familiar adenocarcinomas of the gastrointestinal tract, ranks third in the world in cancer-related deaths. Traditional Chinese medicine can suppress the growth of tumors, and the underlying mechanism may be associated with the tumor microenvironment. Here, we investigated the anti-cancer effects of the Qingrexiaoji recipe on gastric cancer and the underlying molecular mechanism.

Methods:An in vivo nude mouse model was established, and the expression of CD206, CD80, and M2 phenotype-related proteins (Arg-1, Fizz1) was obtained by flow cytometry and western blotting. The expressions of the M2 phenotype-related cytokines were examined by ELISA.

Results:Qingrexiaoji recipe inhibited gastric tumor growth and downregulated the expression of CD206, IFN-γ, IL-13, IL-4, and TNF-α in vivo. Qingrexiaoji recipe deceased M2 phenotypic polarization by upregulating microRNA (miR)-29a-3p level. Luciferase activity assays showed that HDAC4 is a potential target of miR-29a-3p. In cells co-transfected with HDAC4 siRNA and miR-29a-3p inhibitor and treated with IL-4 and Qingrexiaoji recipe, the miR-29a-3p inhibitorinduced increase of M2 phenotypic polarization was reversed.

Conclusion:In summary, these results suggested that the Qingrexiaoji recipe regulated M2 macrophage polarization by regulating miR-29a-3p/HDAC4, providing a different and innovative treatment for gastric cancer.

About the authors

Yiqiong Zhang

Department of First Clinical Medical College, Zhejiang Chinese Medical University

Email: info@benthamscience.net

Luting Chen

Department of Integrated Chinese and Western Medicine, The First People’s Hospital of Wenling

Email: info@benthamscience.net

Yuchang Fei

Department of Integrated Chinese and Western Medicine, The First People’s Hospital of Jiashan

Email: info@benthamscience.net

Peifeng Chen

Department of Oncology, The First Affiliated Hospital of Zhejiang Chinese Medical University

Email: info@benthamscience.net

Lei Pan

Department of Oncology, The First Affiliated Hospital of Zhejiang Chinese Medical University

Author for correspondence.
Email: info@benthamscience.net

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