Long Non-coding RNAs Influence Aging Process of Sciatic Nerves in SD Rats


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Abstract

Objectives:To investigate the long non-coding RNAs (lncRNAs) changes in the sciatic nerve (SN) in Sprague Dawley (SD) rats during aging.

Methods:Eighteen healthy SD rats were selected at the age of 1 month (1M) and 24 months (24M) and SNs were collected. High-throughput transcriptome sequencing and bioinformatics analysis were performed. Protein-protein interaction (PPI) networks and competing endogenous RNA (ceRNA) networks were established according to differentially expressed genes (DEGs).

Result:As the length of lncRNAs increased, its proportion to the total number of lncRNAs decreased. A total of 4079 DElncRNAs were identified in Con vs. 24M. GO analysis was primarily clustered in nerve and lipid metabolism, extracellular matrix, and vascularization-related fields. There were 17 nodes in the PPI network of the target genes of up-regulating genes including Itgb2, Lox, Col11a1, Wnt5a, Kras, etc. Using quantitative RT-PCR, microarray sequencing accuracy was validated. There were 169 nodes constructing the PPI network of down-regulated target genes, mainly including Col1a1, Hmgcs1, Hmgcr. CeRNA interaction networks were constructed.

Conclusion:Lipid metabolism, angiogenesis, and ECM fields might play an important role in the senescence process in SNs. Col3a1, Serpinh1, Hmgcr, and Fdps could be candidates for nerve aging research.

About the authors

Rui Kuang

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Email: info@benthamscience.net

Yi Zhang

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Email: info@benthamscience.net

Guanggeng Wu

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Email: info@benthamscience.net

Zhaowei Zhu

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Email: info@benthamscience.net

Shuqia Xu

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Email: info@benthamscience.net

Xiangxia Liu

Department of Plastic Surgery, University of Tennessee Health Science Center

Author for correspondence.
Email: info@benthamscience.net

Yangbin Xu

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Author for correspondence.
Email: info@benthamscience.net

Yunxiang Luo

Department of Plastic Surgery, First Affiliated Hospital of Sun Yat-sen University

Author for correspondence.
Email: info@benthamscience.net

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