Protective Effects of Liriodendrin on Myocardial Infarction-Induced Fibrosis in Rats via the PI3K/Akt Autophagy Pathway: A Network Pharmacology Study


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Background:Liriodendrin (LIR) has been reported to improve cardiac function in rats following myocardial infarction. However, its role and mechanism in reparative myocardial fibrosis remain unclear.

Methods:In this study, a rat model of myocardial fibrosis was established via left anterior descending artery ligation and randomly divided into three groups (n = 6 per group): sham-operated, myocardial infarction, and LIR intervention (100 mg/kg/day) groups. The pharmacological effects of LIR were assessed using echocardiography, hematoxylin, and eosin (H&E) staining, and Masson staining. Network pharmacology and bioinformatics were utilized to identify potential mechanisms of LIR, which were further validated via western blot analysis.

Results:Our findings demonstrated that LIR improved cardiac function, histology scores, and col lagen volume fraction. Moreover, LIR downregulated the expression of Beclin-1, LC3-II/LC3-I while upregulating the expression of p62, indicating LIR-inhibited autophagy in the heart after myocardial infarction. Further analysis revealed that the PI3K/Akt signaling pathway was significantly enriched and validated by western blot. This analysis suggested that the ratios of p- PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR were significantly increased.

Conclusion:LIR may attenuate myocardial infarction-induced fibrosis in rats by inhibiting excessive myocardial autophagy, with the potential mechanism involving the activation of the PI3K/Akt/mTOR pathway.

Sobre autores

Ping Zhang

Department of Cardiology, Tianjin Nankai Hospital

Email: info@benthamscience.net

Xuanming Liu

Graduate School, Tianjin University of Traditional Chinese Medicine

Email: info@benthamscience.net

Xin Yu

Graduate School, Tianjin University of Traditional Chinese Medicine

Email: info@benthamscience.net

Yuzhen Zhuo

Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair, Tianjin Nankai Hospital

Email: info@benthamscience.net

Dihua Li

Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair, Tianjin Nankai Hospital

Email: info@benthamscience.net

Lei Yang

Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair, Tianjin Nankai Hospital

Autor responsável pela correspondência
Email: info@benthamscience.net

Yanmin Lu

Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair Abdomen Disease Associated Organ Injury and ITCWM Repair, Tianjin Nankai Hospital

Autor responsável pela correspondência
Email: info@benthamscience.net

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