Effects of Dihydroquercetin on the Intensity of Oxydative Stress in Rat Liver Mitochondria at Hypothermia

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Abstract

A decrease in body temperature in homeothermic animals can cause a state of the body called hypothermic. It is accompanied by the development of a number of pathological processes, many of which are associated with mitochondrial dysfunction and the development of oxidative stress. In connection with the widespread introduction of hypothermia into medical practice, the question of the possibility of a regulatory influence on the proxidant-antioxidant status of mitochondria at low body temperatures remains relevant. In recent years, plant polyphenols, in particular dihydroquercetin (DHQ), have gained wide popularity as therapeutic agents with antioxidant and membrane protective effects. In this work, we investigated the effects of DHQ on the intensity of oxidative stress in rat liver mitochondria under moderate hypothermia. It was found that a course (5 days) oral administration of DHA at a dose of 100 mg/kg significantly reduces the levels of LPO and OMP products in the liver mitochondria of control rats, increasing the content of non-enzymatic components of the thiol-disulfide antioxidant system`s. DHQ effectively protects liver mitochondria from the development of oxidative stress during hypothermia, as evidenced by a significant decrease (and in some cases, complete normalization) in the levels of diene conjugates, MDA, Schiff bases and carbonyl groups in a group of animals subjected to hypothermia with prior administration of this polyphenol. At the same time, DHQ significantly increases the levels of glutathione and vitamin E, and also normalizes the content of thiol groups in mitochondrial proteins. In vitro, DHQ exhibits a dose-dependent antioxidant effect, suppressing OMB in mitochondria incubated in Fenton's medium (IC50 = 0.160 mg/ml).

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R. A. Khalilov

Dagestan State University

Email: albina19764@mail.ru
Russian Federation, Makhachkala

A. M. Dzhafarova

Dagestan State University

Author for correspondence.
Email: albina19764@mail.ru
Russian Federation, Makhachkala

Z. G. Rabadanova

Dagestan State University

Email: albina19764@mail.ru
Russian Federation, Makhachkala

M. B. Dzhafarov

Astrakhan State Medical University

Email: albina19764@mail.ru
Russian Federation, Astrakhan

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2. Fig. 1. Correlations between the level of glutathione and the content of sulfhydryl groups in rat liver mitochondrial proteins under various physiological conditions (control, course DHA, short-term hypothermia, moderate hypothermia against the background of course administration of DHA). r = 0.88, p < 0.05.

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3. Fig. 2. The kinetic curve of the dependence of Si/Co (%) on the concentration of DHA (Si is the concentration of carbonyl groups at a given concentration of DHA, Co is the concentration of carbonyl groups in a medium without DHA). The dotted line shows the concentration of DHA, which causes a 50% suppression (IC50) of the intensity of OMB during incubation of isolated mitochondria in the Fenton medium.

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