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Fig. 4. Scheme of the multimerization process: annealing and extension of primer R on DNA template I, leading to the formation of a double-stranded product of length 1L (step 1), formation of the pseudocyclic DNA structure Ini (step 2), elongation of chains in Ini, formation of a double-stranded product of length 2L (steps 3, 4 and 5b) or annealing and elongation of primer R (steps 3, 4 and 5a), formation of template II (step 5a), displacement of DNA template II and annealing of primer F on it (step 6), formation of full-length amplicons with tandem nucleotide sequences (steps i). Primer F and its complementary regions are shown in black, primer R and its complementary regions are shown in gray |