Mechanisms of antioxidant protection of low-density lipoprotein particles against free radical oxidation

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Abstract

It was found that when patients with atherosclerosis are orally administered ubiquinon Q10 (CoQ10), the oxidation (lipohydroperoxide content) of low-density lipoprotein (LDL) particles sharply decreases, which confirms the important role of this natural antioxidant in protecting LDL particles from free radical oxidation in vivo. The influence of lipophilic natural antioxidants ubiquinol Q10 (CoQ10H2) and α-tocopherol (α-TOH) on the kinetic parameters of Cu2+-initiated free radical oxidation of LDL particles was investigated. In this model system, the possible synergism of the antioxidant action of CoQ10H2 and α-TOH is shown. The probable mechanisms of bioregeneration of the lipophilic antioxidants in LDL particles, including regeneration of α-TOH from the tocopheroxyl radical (α-TO•) with the participation of CoQ10H2 and/or ascorbate, are discussed.

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About the authors

V. Z. Lankin

National Medical Research Center of Cardiology Named after Academician E. I. Chazov, Ministry of Health of the Russian Federation

Author for correspondence.
Email: lankin0309@mail.ru
Russian Federation, 121552 Moscow

K. B. Shumaev

National Medical Research Center of Cardiology Named after Academician E. I. Chazov, Ministry of Health of the Russian Federation; Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences

Email: lankin0309@mail.ru
Russian Federation, 121552 Moscow; 119071 Moscow

V. A. Medvedeva

National Medical Research Center of Cardiology Named after Academician E. I. Chazov, Ministry of Health of the Russian Federation

Email: lankin0309@mail.ru
Russian Federation, 121552 Moscow

A. K. Tikhaze

National Medical Research Center of Cardiology Named after Academician E. I. Chazov, Ministry of Health of the Russian Federation

Email: lankin0309@mail.ru
Russian Federation, 121552 Moscow

G. G. Konovalova

National Medical Research Center of Cardiology Named after Academician E. I. Chazov, Ministry of Health of the Russian Federation

Email: lankin0309@mail.ru
Russian Federation, 121552 Moscow

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Supplementary files

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2. Fig. 1. Structural formulas of the studied lipophilic phenolic antioxidants

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3. Fig. 2. Effect of oral administration of ubiquinone Q10 to patients with coronary heart disease on the LOOH content in LDL of blood plasma.

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4. Fig. 3. Effect of different lipophilic antioxidants on the kinetics of LOOH (diene conjugates) accumulation during Cu2+-initiated SRO of particles

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5. Fig. 4. Dependence of the duration of the induction period (τ) of the FRO of LDL particles (according to the accumulation of diene conjugates) on the concentration of added phenolic lipophilic antioxidants:

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6. Fig. 5. Effect of different concentrations of exogenous α-tocopherol, ubiquinol Q10 and skQ1 on the initial rate of Cu2+-initiated lipid FRO in LDL particles

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7. Fig. 6. Change in the initial velocity of Cu2+-initiated FRO in LDL particles upon addition of 10 μM α-tocopherol and different concentrations

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8. Fig. 7. Reduction of free radicals of α-tocopherol (α-TO•) and probucol.

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9. Fig. 8. Hypothetical scheme illustrating possible mechanisms of bioregeneration of CoQ10H2 and α-TON during the process of SRO of LDL particles

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