Novel rhamnose-containing glycopolymers from the cell wall of Rathayibacter festucae VKM Ac-1390T

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Abstract

Rathayibacter festucae VKM Ac-1390T (family Microbacteriaceae, class Actinomycetes) contains three glycopolymers in the cell wall. The structures of glycopolymers established by chemical and NMR spectroscopy methods. One of them, rhamnomannan, built from repeating tetrasaccharide units carrying side xylopyranose residues, →2)-α-[β-D-Xylp-(1→3)]-D-Rhap-(1→3)-α-D-Manp-(1→2)-α-D-Rhap-(1→3)-α-D-Manp-(1→. The second polymer, found in minor amounts in the strain studied, is rhamnan, →2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→. The third polymer is teichuronic acid, acetalated with pyruvic acid, →2)-α-[4,6-S-Pyr]-D-Manp-(1→4)-α-L-Rhap-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp-(1→4)-β-D-GlcpA-(1→. The structures of rhamnomannan and teichuronic acid are new for Ratayibacter and prokaryotes in a whole. The results of the present study expand our understanding of the structural diversity of microbial glycopolymers and are consistent with the data on the specificity of the structures and composition of glycopolymers for species of the genus Rathayibacter described previously.

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About the authors

A. S. Shashkov

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: potekhina56@mail.ru
Russian Federation, 119991 Moscow

N. V. Potekhina

Lomonosov Moscow State University

Author for correspondence.
Email: potekhina56@mail.ru

Faculty of Biology

Russian Federation, 119234 Moscow

E. M. Tul’skaya

Lomonosov Moscow State University

Email: potekhina56@mail.ru

Faculty of Biology

Russian Federation, 119234 Moscow

A. S. Dmitrenok

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: potekhina56@mail.ru
Russian Federation, 119991 Moscow

S. N. Senchenkova

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: potekhina56@mail.ru
Russian Federation, 119991 Moscow

L. V. Dorofeeva

All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

Email: potekhina56@mail.ru
Russian Federation, 142290 Pushchino, Moscow Region

L. I. Evtushenko

All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

Email: potekhina56@mail.ru
Russian Federation, 142290 Pushchino, Moscow Region

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Supplementary files

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2. Fig. 1. 13C NMR spectra of polysaccharides from the cell wall of R. festucaе BKM Ac-1390T: total preparation (a); neutral fraction (b); acidic fraction (c)

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3. Fig. 2. Portions of the 1H,13C HSQC spectrum of the neutral polysaccharide(s) from the cell wall of R. festucaе BKM Ac-1390T. Portions of the 1H and 13C NMR spectra are shown along the horizontal and vertical axes, respectively. Arabic numerals refer to carbon atoms in residues as designated in Table 1

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4. Fig. 3. Part of the 1H,1H ROESY spectrum of neutral polysaccharide(s) from the cell wall of R. festucaе BKM Ac-1390T. Arabic numerals refer to protons in residues as indicated in Table 1. Slashes refer to correlation peaks between residues.

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5. Рис. 4. Части спектра 1H,13C HMBC нейтрального полисахарида(ов) из клеточной стенки R. festucaе ВKM Ac-1390T. Заглавные латинские буквы относятся к остаткам, как обозначено в табл. 1. Арабские цифры перед косой чертой относятся к протонам, а после косой черты – к атомам углерода в соответствующих остатках

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6. Fig. 5. Portions of the 1H,13C HSQC spectrum of the acidic polysaccharide from the cell wall of R. festucaе BKM Ac-1390T. Portions of the 1H and 13C NMR spectra are shown along the horizontal and vertical axes, respectively. Arabic numerals refer to carbon atoms in residues as designated in Table 1

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7. Fig. 6. Part of the 1H,1H ROESY spectrum of the acidic polysaccharide from the cell wall of R. festucaе BKM Ac-1390T. Arabic numerals refer to protons in residues as indicated in Table 1. Slashes refer to correlation peaks between residues.

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8. Fig. 7. Part of the 1H,13C HMBC spectrum of the acidic polysaccharide from the cell wall of R. festucaе BKM Ac-1390T. Capital letters refer to residues as indicated in Table 1. Arabic numerals before the slash refer to protons, and after the slash to carbon atoms in the corresponding residues.

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