Serum exosome protein composition in ischemic stroke patients is associated with cognitive impairment in the post-stroke period

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Abstract

Ischemic stroke (IS) and subsequent neuropsychiatric disorders are among the leading causes of disability worldwide. Several strategies have been previously proposed to utilize exosomes to assess the risk of IS-related diseases. The aim of this work was to evaluate serum exosome proteins of IS patients in the chronic post-stroke period and to search for their associations with the development of post-stroke moderate cognitive impairment (MCI). Comparative quantitative proteomic analysis of serum exosomes of patients without poststroke MCI (19 patients mean age 52.0 ± 8.1 years) and patients with post-stroke MCI (11 patients, mean age 64.8 ± 5.6 years) revealed significant differences in the levels of 62 proteins out of 186 identified. Increased levels of proteins associated with the immune system and decreased levels of proteins involved in lipid metabolism were observed in patients with MCI compared to patients without MCI in the chronic post-stroke period. The obtained data suggest that a higher level of immune system activation in patients in the distant period after IS may be one of the risk factors for the development of post-stroke cognitive disorders and suggest the participation of exosomal transport in these processes.

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About the authors

T. A. Druzhkova

Moscow Research and Clinical Center for Neuropsychiatry

Email: nata_gul@ihna.ru
Russian Federation, 115419 Moscow

M. Yu. Zhanina

Moscow Research and Clinical Center for Neuropsychiatry; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences

Email: nata_gul@ihna.ru

Department of Functional Biochemistry of Nervous System

Russian Federation, 115419 Moscow; 117485 Moscow

E. E. Vladimirova

M. P. Konchalovsky City Clinical Hospital

Email: nata_gul@ihna.ru
Russian Federation, 124489 Moscow

A. B. Guekht

Moscow Research and Clinical Center for Neuropsychiatry; Pirogov Russian National Research Medical University

Email: nata_gul@ihna.ru
Russian Federation, 115419 Moscow; 119049 Moscow

N. V. Gulyaeva

Moscow Research and Clinical Center for Neuropsychiatry; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences

Author for correspondence.
Email: nata_gul@ihna.ru

Department of Functional Biochemistry of Nervous System

Russian Federation, 115419 Moscow; 117485 Moscow

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2. Fig. 1. Heatmap showing the results of hierarchical clustering of samples and protein profiles of all patients after AI. Clustering of protein expression has been shown in groups of patients without and with HCN

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