Characteristics of PRIMPOL knockout А549 cell response to genоtoxic stress

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Abstract

Human DNA primase/polymerase PrimPol synthesizes DNA primers de novo after replication fork stalling at damaged DNA sites, contributing to DNA damage tolerance. The contribution of PrimPol in response to the various types of DNA damage is not fully understood. We obtained the lung carcinoma cells A549 with PRIMPOL knockout and characterized its response to DNA damage caused by hydrogen peroxide, methylmethanesulfonate (MMS), cisplatin, bleomycin and ionizing radiation. Knockout of PRIMPOL reduced the number of proliferating cells and cells in G2 phase after treatment with MMS, caused a more pronounced delay of cisplatin-treated cells in S phase. A significant increase in the proportion of apoptotic cells was noted in PRIMPOL-/- cells in response to ionizing radiation at a dose of 10 Gy, while the proportion of cells prone to necroptosis increased significantly in both parental and knockout cells at any radiation dose. Under conditions of oxidative stress stimulated by hydrogen peroxide, PRIMPOL knockout increased cell viability, measured by the MTT method. The data obtained indicate the involvement of PRIMPOL in modulating stress-adaptive responses to various types of genotoxic stress.

About the authors

A. S Gromova

Institute of Molecular Genetics, National Research Center “Kurchatov Institute”;Institute of Gene Biology, Russian Academy of Sciences

123182 Moscow, Russia;119334 Moscow, Russia

E. O Boldinova

Institute of Molecular Genetics, National Research Center “Kurchatov Institute”;Institute of Gene Biology, Russian Academy of Sciences

123182 Moscow, Russia;119334 Moscow, Russia

D. V Kim

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences;Novosibirsk State University

630090 Novosibirsk, Russia;630090 Novosibirsk, Russia

R. N Chuprov-netochin

Moscow Institute of Physics and Technology

141700 Dolgoprudny, Moscow Region, Russia

S. V Leonov

Moscow Institute of Physics and Technology;Institute of Cell Biophysics of the Russian Academy of Sciences

141700 Dolgoprudny, Moscow Region, Russia;142290 Pushchino, Russia

M. V Pustovalova

Moscow Institute of Physics and Technology

141700 Dolgoprudny, Moscow Region, Russia

D. O Zharkov

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences;Novosibirsk State University

Email: dzharkov@niboch.nsc.ru
630090 Novosibirsk, Russia;630090 Novosibirsk, Russia

A. V Makarova

Institute of Molecular Genetics, National Research Center “Kurchatov Institute”;Institute of Gene Biology, Russian Academy of Sciences

Email: amakarova-img@yandex.ru
123182 Moscow, Russia;119334 Moscow, Russia

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