Physical and chemical analysis of the lipofuscin granule bisretinoid photodestruction products from retinal pigment epithelium cells of the eye

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Resumo

In this work, the mechanisms of formation of the bisretinoid oxidation products in lipofuscin granules isolated from the retinal pigment epithelium cells of the human eye have been studied. The physico-chemical characteristics of the bisretinoid photooxidation products are described. The methods of IR spectroscopy, Raman spectroscopy, fluorescence spectroscopy, scanning confocal microscopy, time-of-flight mass spectrometry of secondary ions (TOF.SIMS) and HPLC were used for the study. The properties of the products of photooxidation and degradation of the fluorophore of lipofuscin granules, including synthetic N-retinylidene-N-retinylethanolamine (A2E), are described in detail. It has been shown that the products of oxidative degradation of lipofuscin granules are similar to the products of photooxidation of the main bisretinoid of lipofuscin granules – A2E. These data are important both for understanding the mechanisms of formation of cytotoxic products in lipofuscin granules and for establishing their chemical nature.

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Sobre autores

M. Yakovleva

Emanuel Institute of Biochemical Physics RAS

Autor responsável pela correspondência
Email: lina.invers@gmail.com
Rússia, Moscow

A. Vasin

Semenov Federal Research Center for Chemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

A. Dontsov

Emanuel Institute of Biochemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

A. Gulin

Semenov Federal Research Center for Chemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

A. Aybush

Semenov Federal Research Center for Chemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

A. Astafiev

Semenov Federal Research Center for Chemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

A. Shakhov

Semenov Federal Research Center for Chemical Physics RAS

Email: lina.invers@gmail.com
Rússia, Moscow

T. Feldman

Emanuel Institute of Biochemical Physics RAS; Lomonosov Moscow State University

Email: lina.invers@gmail.com
Rússia, Moscow; Moscow

M. Ostrovsky

Emanuel Institute of Biochemical Physics RAS; Lomonosov Moscow State University

Email: lina.invers@gmail.com
Rússia, Moscow; Moscow

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2. Fig. 1. a – Fluorescence spectra of chloroform extracts from suspensions of native lipofuscin granules (1) and irradiated with visible light (2) with a wavelength of 488 nm; b – chromatograms of chloroform extract from LG suspension: 1 – non-irradiated LG, 2 – LG after irradiation with visible light. Detection – by absorption at a wavelength of 430 nm.

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3. Fig. 2. 3D fluorescence spectra: a – fluorescence profiles of A2E; b – fluorescence profiles of A2E irradiated with visible light; c – fluorescence profiles of LG suspension; g – fluorescence profiles of LG suspension irradiated with visible light; d – fluorescence profiles of chloroform extract of LG; e – fluorescence profiles of chloroform extract of LG irradiated with visible light.

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4. Fig. 3. Raman spectra of samples before (black spectrum) and after exposure to visible light (gray spectrum): a – suspension of lipofuscin granules, b – chloroform extracts from LG, c – synthetic A2E.

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5. Fig. 4. IR spectra of samples before (black spectrum) and after exposure to visible light (gray spectrum): a – suspension of lipofuscin granules, b – chloroform extracts from LG, c – synthetic A2E.

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6. Fig. 5. Histograms of mass spectrometric data before (dark columns) and after exposure to light (light columns) on the studied samples: a – LG suspension, b – chloroform extracts from LG, c – synthetic A2E. For clarity, the ion intensities were normalized to the corresponding average ion intensity of the dark sample.

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