Synthesis of peptide fragments spike glycoprotein SARS-CoV-2 and studying their binding with human blood cells

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Resumo

A new method was tested that allows to evaluate activation of the Spike surface protein of SARS-CoV-2 blood leukocytes by synthetic peptides, fragments Lys-Ile-Ala-Asp-Tyr-Asn-Tyr-Lys-Leu (417–425 аа) and Val-Arg-Gln-Ala-Pro-Asn-Gly-Gln-Thr (407–415 аа). It was found that these peptides, regardless of the HLA-A phenotype of the subjects, could bind to leukocytes, which indicates the universality of reactions to peptides, especially in innate immune cells. It was shown that the Lys-Ile-Ala-Asp-Tyr-Asn-Tyr-Lys-Leu peptide contacted the blood leukocytes of the subjects, activated the lymphocytes and basophils of the subjects (statistically significant), which was confirmed by an increase in gamma interferon compared to the Val-Arg-Gln-Ala-Pro-Asn-Gly-Gln-Thr. Thus, this work demonstrates an approach to creating a new peptide vaccine against COVID-19 at the stage of in vitro research.

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Sobre autores

О. Gribovskaya

Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus

Autor responsável pela correspondência
Email: olymelnik@yandex.ru
Belarus, ul. Akad. Kuprevicha 5/2, Minsk, 220084

V. Yanchenko

Vitebsk State medical University

Email: olymelnik@yandex.ru
Belarus, pr. Frunze 27, Vitebsk, 210023

A. Tsygankov

Vitebsk State medical University

Email: olymelnik@yandex.ru
Rússia, pr. Frunze 27, Vitebsk, 210023

V. Martinovich

Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus

Email: olymelnik@yandex.ru
Rússia, ul. Akad. Kuprevicha 5/2, Minsk, 220084

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2. Scheme 1. Synthesis of peptide (IX).

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3. Scheme 2. Synthesis of peptide (XVIII).

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