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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="other" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Epidemiology and Infectious Diseases</journal-id><journal-title-group><journal-title xml:lang="en">Epidemiology and Infectious Diseases</journal-title><trans-title-group xml:lang="ru"><trans-title>Эпидемиология и инфекционные болезни</trans-title></trans-title-group></journal-title-group><issn publication-format="print">3034-2007</issn><issn publication-format="electronic">3034-2015</issn><publisher><publisher-name xml:lang="en">Eco-Vector</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">702173</article-id><article-id pub-id-type="doi">10.17816/EID702173</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Original study articles</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Оригинальные исследования</subject></subj-group><subj-group subj-group-type="article-type"><subject></subject></subj-group></article-categories><title-group><article-title xml:lang="en">STUDY OF THE ACTIVITY OF A POSTBIOTIC BASED ON THE ENTEROCOCCUS FAECIUM L-3 STRAIN TO OPTIMIZE THE QUALITATIVE AND QUANTITATIVE COMPOSITION OF HUMAN MICROBIOTA UNDER CONDITIONS OF LONG-TERM ISOLATION IN CONFINED HABITAT</article-title><trans-title-group xml:lang="ru"><trans-title>ИССЛЕДОВАНИЕ АКТИВНОСТИ ПОСТБИОТИКА НА ОСНОВЕ ШТАММА ENTEROCOCCUS FAECIUM L-3 ДЛЯ ОПТИМИЗАЦИИ КАЧЕСТВЕННОГО И КОЛИЧЕСТВЕННОГО СОСТАВА МИКРОБИОТЫ ЧЕЛОВЕКА В УСЛОВИЯХ ДЛИТЕЛЬНОЙ ИЗОЛЯЦИИ В ГЕРМОПОМЕЩЕНИИ</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3896-5003</contrib-id><name-alternatives><name xml:lang="en"><surname>Ilyin</surname><given-names>Vyacheslav</given-names></name><name xml:lang="ru"><surname>Ильин</surname><given-names>Вячеслав Константинович</given-names></name></name-alternatives><email>piton2004@bk.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2312-5589</contrib-id><name-alternatives><name xml:lang="en"><surname>Suvorov</surname><given-names>Aleksandr</given-names></name><name xml:lang="ru"><surname>Суворов</surname><given-names>Александр Николаевич</given-names></name></name-alternatives><email>a.n.suvorov@spbu.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7854-3513</contrib-id><name-alternatives><name xml:lang="en"><surname>Kostyuchenko</surname><given-names>Marina</given-names></name><name xml:lang="ru"><surname>Костюченко</surname><given-names>Марина Николаевна</given-names></name></name-alternatives><email>m.kostyuchenko@gosniihp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4026-5789</contrib-id><name-alternatives><name xml:lang="en"><surname>Martirosian</surname><given-names>Vladimir</given-names></name><name xml:lang="ru"><surname>Мартиросян</surname><given-names>Владимир Викторович</given-names></name></name-alternatives><email>v.martirosyan@gosniihp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6662-7530</contrib-id><name-alternatives><name xml:lang="en"><surname>Tyurina</surname><given-names>Olga</given-names></name><name xml:lang="ru"><surname>Тюрина</surname><given-names>Ольга Евгеньевна</given-names></name></name-alternatives><email>oeturina@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Nosova</surname><given-names>Marina</given-names></name><name xml:lang="ru"><surname>Носова</surname><given-names>Марина Владимировна</given-names></name></name-alternatives><email>m.nosova@gosniihp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8485-4470</contrib-id><name-alternatives><name xml:lang="en"><surname>Usanova</surname><given-names>Nonna</given-names></name><name xml:lang="ru"><surname>Усанова</surname><given-names>Нонна Альбертовна</given-names></name></name-alternatives><email>usanova@imbp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9461-6986</contrib-id><name-alternatives><name xml:lang="en"><surname>Morozova</surname><given-names>Julia</given-names></name><name xml:lang="ru"><surname>Морозова</surname><given-names>Юлия Алексеевна</given-names></name></name-alternatives><email>morozova@imbp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Alyohina</surname><given-names>Galina</given-names></name><name xml:lang="ru"><surname>Алёхина</surname><given-names>Галина Геннадьевна</given-names></name></name-alternatives><email>a.n.suvorov@spbu.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9540-4769</contrib-id><name-alternatives><name xml:lang="en"><surname>Starkova</surname><given-names>Liubov</given-names></name><name xml:lang="ru"><surname>Старкова</surname><given-names>Любовь Валентиновна</given-names></name></name-alternatives><email>starkova@imbp.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Parfenova</surname><given-names>Angelica</given-names></name><name xml:lang="ru"><surname>Парфенова</surname><given-names>Анжелика Анатольевна</given-names></name></name-alternatives><email>angelikaparfenova3@gmail.com</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6065-2295</contrib-id><name-alternatives><name xml:lang="en"><surname>Vilenskii</surname><given-names>Igor</given-names></name><name xml:lang="ru"><surname>Виленский</surname><given-names>Игорь Леонидович</given-names></name></name-alternatives><email>medosmotr26@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0007-1349-9882</contrib-id><contrib-id contrib-id-type="spin">9126-1010</contrib-id><name-alternatives><name xml:lang="en"><surname>Alieva</surname><given-names>Elena</given-names></name><name xml:lang="ru"><surname>Алиева</surname><given-names>Елена Васильевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Professor of the Department of Medical Microbiology named after Academician Z.V. Ermolyeva</p></bio><bio xml:lang="ru"><p>профессор кафедры медицинской микробиологии имени академика З.В. Ермольевой</p></bio><email>elalieva.ru@mail.ru</email><xref ref-type="aff" rid="aff2"/></contrib></contrib-group><aff id="aff1"><institution></institution></aff><aff-alternatives id="aff2"><aff><institution xml:lang="en">Federal State Budgetary Educational Institution of Additional Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Health of the Russian Federation</institution></aff><aff><institution xml:lang="ru">ФГБОУ ДПО «Российская медицинская академия непрерывного профессионального образования» Министерства здравоохранения РФ</institution></aff></aff-alternatives><pub-date date-type="preprint" iso-8601-date="2026-07-13" publication-format="electronic"><day>13</day><month>07</month><year>2026</year></pub-date><volume>31</volume><issue>2</issue><issue-title xml:lang="ru"/><history><date date-type="received" iso-8601-date="2026-02-04"><day>04</day><month>02</month><year>2026</year></date><date date-type="accepted" iso-8601-date="2026-03-22"><day>22</day><month>03</month><year>2026</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; , Eco-vector</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; , Эко-вектор</copyright-statement><copyright-holder xml:lang="en">Eco-vector</copyright-holder><copyright-holder xml:lang="ru">Эко-вектор</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/" start_date="2029-03-22"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://eco-vector.com/for_authors.php#07</ali:license_ref></license></permissions><self-uri xlink:href="https://rjeid.com/1560-9529/article/view/702173">https://rjeid.com/1560-9529/article/view/702173</self-uri><abstract xml:lang="en"><p>The pilot study evaluated the effect of using a postbiotic in the form of bread with an inactivated culture of the probiotic strain Enterococcus faecium L3. on the quantitative and qualitative parameters of the intestinal, nasal and pharyngeal microbiome of testers in 60-day isolation. The results of the study indicate an increase in positive changes in the composition of the intestinal microbiota: trends towards an increase in the number of representatives of protective microorganisms - bifidobacteria, lactobacilli, and in some cases, enterococci against the background of numerical suppression of enterobacteria, staphylococci and yeast.</p></abstract><trans-abstract xml:lang="ru"><p>В пилотном исследовании произведена оценка влияния использования постбиотика в виде хлеба с инактивированной культурой пробиотического штамма Enterococcus faecium L3. на количественные и качественные показатели микробиома кишечника, носа и глотки испытателей в условиях 60- суточной изоляции. Результаты исследования свидетельствуют о нарастании позитивных сдвигов в составе микробиоты кишечника: тенденции к увеличению количества представителей протективных микроорганизмов - бифидобактерий, лактобацилл, и в ряде случаев, энтерококков на фоне численного угнетения энтеробактерий, стафилококков и дрожжей.</p> <p> </p></trans-abstract><kwd-group xml:lang="en"><kwd>MIcrobiota</kwd><kwd>postbiotic</kwd><kwd>enterococcus faecium l3</kwd><kwd>insulation.</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>Микробиота</kwd><kwd>постбиотик</kwd><kwd>enterococcus faecium l3</kwd><kwd>изоляция.</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution xml:lang="ru">РАН и Минобрнауки</institution></institution-wrap><institution-wrap><institution xml:lang="en">RAS and the Ministry of Education and Science</institution></institution-wrap></funding-source><award-id>FRFM 2024-0035</award-id></award-group><funding-statement xml:lang="en">The work was carried out within the framework of the topic of the Russian Academy of Sciences and the Ministry of Education and Science FRFM 2024-0035 "Research of ways to correct the microbial status of humans and optimize the physiological state of their digestive system in an artificial environment. Investigation of ways and means of preserving the phototrophic link from microbial damage. Study of adaptation and intrapopulation variability of microorganisms in an artificial habitat and under extreme conditions"</funding-statement><funding-statement xml:lang="ru">Работа выполнена в рамках темы РАН и Минобрнауки FRFM 2024-0035 «Исследование способов коррекции микробного статуса человека и оптимизации физиологического состояния его пищеварительной системы в искусственной среде обитания. Исследование способов и средств сохранения фототрофного звена от микробных поражений. Исследование адаптации и внутрипопуляционной изменчивости микроорганизмов в условиях искусственной среды обитания и при экстремальных воздействиях»</funding-statement></funding-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>1. Ma L, Tu H, Chen T. Postbiotics in Human Health: A Narrative Review. Nutrients. 2023 Jan 6;15(2):291. doi: 10.3390/nu15020291. 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