Epidemiology and Infectious DiseasesEpidemiology and Infectious DiseasesЭпидемиология и инфекционные болезни3034-20073034-2015Eco-Vector51634910.17816/EID516349Original study articlesОригинальные исследованияResearch ArticleStreptococcus agalactiae in asymptomatic bacteriuria in pregnant womenStreptococcus agalactiae при бессимптомной бактериурии у беременныхhttps://orcid.org/0009-0003-2574-62047849-2212SemechkinNikolai V.СемечкинНиколай Викторович
Russian Federation

MD, Cand. Sci. (Med.)

Кандидат медицинских наук

nykolay1@gmail.comhttps://orcid.org/0009-0006-8092-26471896-8240RomanovVitaly A.РомановВиталий Александрович
Russian Federation

MD, Dr. Sci. (Med), Professor

доктор медицинских наук, профессор

mister.bandicut@gmail.comhttps://orcid.org/0009-0001-8841-6275DanilikOleg N.ДаниликОлег Николаевич
Russian Federation

student

Студент

oleg.danilik7@gmail.comhttps://orcid.org/0009-0005-4512-82991085-0928NovosadovaIrina G.НовосадоваИрина Григорьевна
Russian Federation

MD

заведующая микробиологической лабораторией

novosadova@mail.ruhttps://orcid.org/0000-0003-4691-648XErshovaMarina G.ЕршоваМарина Геннадьевна
Russian Federation

MD

Заведующая микробиологической лабораторией

marierchowa2013@yandex.ruAkentyevaSvetlana A.АкентьеваСветлана Александровна
Russian Federation

MD

врач микробиологической лаборатории

lana2500@mail.ruYaroslavl State Medical UniversityЯрославский государственный медицинский университетBalashikha Regional HospitalБалашихинская областная больницаInfectious Clinical HospitalИнфекционная клиническая больница11102023051120232852892952806202305092023Copyright ©; 2023, Eco-vectorCopyright ©; 2023, Эко-вектор2023Eco-vectorЭко-векторhttps://rjeid.com/1560-9529/article/view/516349

BACKGROUND: Asymptomatic bacteriuria caused by group B gram-positive streptococcus, Streptococcus agalactiae, in pregnant women is a leading cause of sepsis in newborns and young children, transmitted from their mothers. In the present study, the frequency of S. agalactiae inoculation from the urine of pregnant women with asymptomatic bacteriuria was studied with the determination of the sensitivity of isolated strains to antibiotics.

AIMS: This study aimed to investigate the inoculation of group B streptococcus in asymptomatic bacteriuria of pregnant women and to determine the sensitivity of the isolated cultures of the pathogen to the main antibiotics.

MATERIALS AND METHODS: The analysis involved bacteriological studies of 2,559 urine samples collected from pregnant women. Urine was inoculated on UriSelect 4 Medium chromogenic medium with subsequent identification of S. agalactiae using a strepto-latex test or a CAMP test with a Staphylococcus aureus culture. The sensitivity of microorganisms to antibacterial drugs was determined using the disk diffusion method. Data analysis was conducted using the Statistica 12.0 software package.

RESULTS: Out of 2,559 urine samples studied, asymptomatic bacteriuria was detected in 317 samples (12.4%), and 330 strains of microorganisms were isolated. S. agalactiae was isolated by culture from 108 pregnant women (4.2% of the total subjects), with 50 cases (16%) showing asymptomatic bacteriuria caused by S. agalactiae. The high significance of Escherichia coli in asymptomatic bacteriuria in pregnant women was shown, with a lesser extent of involvement observed with Enterococcus spp. Furthermore, associations of S. agalactiae with other microorganisms were identified. The high sensitivity of S. agalactiae strains to clindamycin, to a lesser extent to levofloxacin, chloramphenicol, and, especially, to erythromycin, has been demonstrated.

CONCLUSIONS: The study revealed a high culturability of group B streptococcus in asymptomatic bacteriuria in pregnant women. Low sensitivity of isolated streptococci to erythromycin, moderate sensitivity to levofloxacin and chloramphenicol, and high sensitivity to clindamycin were also revealed.

Обоснование. Бессимптомная бактериурия у беременных, вызванная грамположительными стрептококками Streptococcus agalactiae группы B, является одной из причин сепсиса у новорождённых и детей раннего возраста в результате их инфицирования собственными матерями. В настоящем исследовании изучена частота высеваемости S. agalactiae из мочи беременных при бессимптомной бактериурии с определением чувствительности выделенных штаммов к антибиотикам.

Цель исследования — изучение высеваемости стрептококка группы В при бессимптомной бактериурии беременных и определение чувствительности выделенных культур возбудителя к основным антибиотикам.

Материалы м методы. В статье проведён анализ результатов бактериологических исследований 2559 образцов мочи от беременных. Мочу засевали на хромогенную среду UriSelect 4 Medium с последующей идентификацией S. agalactiae с помощью стрепто-латекс-теста либо CAMP-теста с культурой Staphylococcus aureus и определением чувствительности микроорганизмов к антибактериальным препаратам диско-диффузионным методом. Статистический анализ проводился на основе вычисления показателя χ2 с применением программного пакета Statistica 12.0.

Результаты. Из исследуемых 2559 образцов мочи в 317 образцах (12,4%) выявлена бессимптомная бактериурия, изолировано 330 штаммов микроорганизмов. S. agalactiae выделен культуральным методом у 108 беременных (4,2% общего числа исследуемых), при этом в 50 случаях (16%) выявлена бессимптомная бактериурия, вызванная S. agalactiae. Показана высокая значимость Escherichia coli при бессимптомной бактериурии у беременных, в меньшей мере — Enterococcus spp. Обнаружены также ассоциации S. agalactiae с иными микроорганизмами. Продемонстрирована высокая чувствительность штаммов S. agalactiae к клиндамицину, в меньшей степени — к левофлоксацину, хлорамфениколу и особенно к эритромицину.

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