New Features of Pectobacterium atrosepticum Virulence Factors

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

The work analyzed nine strains of Pectobacterium atrosepticum (Pca) and obtained new information about the properties of the pathogen and the regulation of its main virulence factors. Anionic groups (PO34–) were identified in exopolysaccharides (EPS) of Pca, which differ in quantity among different strains and may contribute to the formation of denser biofilms. Most Pca strains inhibited the growth of potato plants in vitro to varying degrees, but one of them (strain 426) significantly activated this indicator in plants of the resistant potato variety Lugovskoy. Based on the totality of symptoms of the disease (chlorosis, necrosis, wilt, inhibition of growth rate), all strains of P. atrosepticum were divided according to the degree of virulence. In addition, it was shown that incubation of bacteria with a homogenate from potato plants in vitro modulated the activity of pectinase Pca: a homogenate from plants of the resistant potato variety Lugovskoy inhibited the activity of exopectinase, and from the susceptible variety Lugovskoy activated it. At the same time, the exopectinase activity of the avirulent strain 426 was inhibited to the greatest extent. It is assumed that this was the reason for stimulating the growth of potato plants in vitro of a resistant variety.

Толық мәтін

Рұқсат жабық

Авторлар туралы

L. Lomovatskaya

Siberian Institute of Plant Physiology, Biochemistry Siberian Branch of the Russian Academy of Science

Хат алмасуға жауапты Автор.
Email: LidaL@sifibr.irk.ru
Ресей, Irkutsk, 664033

A. Goncharova

Siberian Institute of Plant Physiology, Biochemistry Siberian Branch of the Russian Academy of Science

Email: LidaL@sifibr.irk.ru
Ресей, Irkutsk, 664033

Әдебиет тізімі

  1. Arif M., Czajkowski R., Chapman T.A. // Front. Plant Sci. 2022. V. 13. P. 1–4.
  2. Sledz M., Pomagruk A.M., Zukowska D., Wensierska W.B., Zoledowska S., Sledz E.L. // Eur. J. Plant Pathol 2023. V. 167. P. 99–121. https://doi.org/10.1007/s10658-023-02687-y
  3. Murata H.,Chatterjef A., Liu Y., Chatterjef A.K. // Appl. Environ. Microbiol 1994. V. 60. № 9. P. 3150–3159.
  4. Николайчик Е.А., Лагоненко А.Л., Валентович Л.Н., Лешкович И.И., Овчинникова Т.В., Присяжненко О.К. и др. // Вестник БГУ. 2006. Сер. 2. № 3. С. 32–37.
  5. Николайчик Е.А. // Труды БГУ. 2012. Т. 7. C. 43–55.
  6. Дюбо Ю.В., Николайчик Е.А. // Молекулярная и прикладная генетика. 2018. Т. 24. С. 37–43.
  7. Gorshkov V., Islamov B., Mikshina P.,Petrova O., Burygin G., Sigida E. et al. // Glycobiology. 2017. V. 27. № 11. Р. 1016–1026. https://doi.org/10.1093/glycob/cwx069
  8. Zheng C.,Wu W., Wu G.,Wang P. // Marine Drugs. 2022. V. 20. P. 512. https://doi.org/10.3390/md20080512.
  9. Луппа Х. Основы гистохимии. М.: Мир, 1980. 343 c.
  10. Ломоватская Л.А., Макарова Л.Е., Кузакова О.В., Романенко А.С., Гончарова А.М. // Прикл. биохимия и микробиология. 2016. Т. 52. № 3.С. 306–311. https://doi.org/10.7868/s0555109916030107
  11. Murashige T., Skoog F. // Physiol. Plant.1962. V. 15. P. 473–497.
  12. Вешняков В.А., Хабаров Ю.Г., Камакина Н.Д. // Химия растительного сырья. 2008. № 4. С. 47–50.
  13. Серегина Н.В., Честнова Т.В., Жеребцова В.А., Хромушин В.А. // Вестник новых медицинских технологий. 2008. Т. 15. № 3. С. 175–177.
  14. Николайчик Е.А, Овчинникова Т.В., Валентович Л.Н., Губич О.И., Шолух М.В., Евтушенков А.Н.// Доклады Национальной академии наук Беларуси. 2005. Т. 45. № 5. С. 81–85.
  15. Tsers I., Parfirova O., Moruzhenkova V., Petrova O., Gogoleva N., Vorob’ev V. et al. // Int. J. Mol. Sci. 2023. V. 24. P. 1–18. https://doi.org/10.3390/ijms241713283
  16. Islamov B., Petrova O., Mikshina P., Kadyirov A., Vorob’ev V., Gogolev Y., Gorshkov V. // Int. J. Mol. Sci. 2021. V. 22. P. 1–16. https://doi.org/10.3390/ijms222312781
  17. Karatan E., Watnick P. // Microbiol. Mol. Biol. Rev. 2009. V. 73. № 2. P. 310–347. https://doi.org/10.1128/MMBR.00041-0810.1128/MMBR.00041-08
  18. Ломоватская Л.А., Романенко А.С., Криволапова Н.В., Копытчук В.Н., Саляев Р.К. // Доклады Академии Наук. 2007. Т. 413. №. 3. С. 420–423.
  19. Indrayati A., Yurina V., Pitaya L.A., Retnoningrum D.S. // MicrobiologyIndonesia. 2011. V. 5. № 2. P. 88–93. https://doi.org/10.5454/mi.5.2.6
  20. Gorshkov V.Y., Daminova A.G., Mikshina P.V., Petrova O.E., Ageeva M.V., Salnikov V.V. et al. // Plant Biol. 2016. V. 4. P. 609–617. https://doi.org/10.1111/plb.12448
  21. Mallick T., Mishra R., Mohanty S., Joshi R.K. // Plant Рathol. J. 2022. V. 38..№ 2. P. 102–114. https://doi.org/10.5423/PPJ.OA.12.2021.0190
  22. Davidsson P.R., Kariola T., Niemi O., Palva E.T. // Front. Plant Sci. 2013. V. 4. Article 191. P. 1–13. https://doi.org/10.3389/fpls.2013.00191
  23. Agyemang P.A., Kabir Md.N., Kersey C.M., Dumenyo C.K. // Horticulturae. 2020. V. 6. P. 13. https://doi.org/10.3390/horticulturae6010013
  24. Joshi J.R., Paudel D., Eddy E., Charkowski A.O., Heuberger A.L // Front. Plant Sci. 2024. V. 15. https://doi.org/10.3389/fpls.2024.1336513
  25. Joshi J.R., Yao l., Charkowski A.O., Heuberger A.L. // Mol. Plant-Microbe Interactions. 2021. V. 34. № 1. P. 100–109. https://doi.org/10.1094/MPMI-08-20-0224-R

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу
2. 1. Effect of potato plant homogenate in vitro on pectinase activity (% of control) of P. atrosepticum strains 22 (1) and 426 (2).

Жүктеу (72KB)
Метадеректер
3. 2. The effect of inoculation with various P. atrosepticum strains on the average growth rate (V, % of control) of potato plants of the Lugovskaya (1) and Lukyanovsky (2) varieties in vitro.

Жүктеу (53KB)
Метадеректер

© Russian Academy of Sciences, 2025